900MHz NMR for Structural Biology in Chicago

芝加哥结构生物学 900MHz NMR

• 批准号: 7116345
• 负责人: PETER G.W. GETTINS
• 金额: $ 24.68万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116345
• 关键词: biomedical equipment resource; cryoscience; nuclear magnetic resonance spectroscopy; structural biology

DESCRIPTION (provided by applicant): The University of Illinois at Chicago (UIC), in collaboration with other major Chicago and Chicago area research institutions, namely Northwestern University, the University of Chicago, Loyola University, and the Medical College of Wisconsin, seek funds for purchase of a narrow bore 21Tesla (900MHz) NMR spectrometer and cryoprobe for carrying out, in solution, high resolution studies on proteins and protein complexes of biomedical importance that demand use of the highest available magnetic field strength. The spectrometer will be housed in a purpose-built, newly-constructed, freestanding structural biology instrumentation building on the Medical Center campus of the University of Illinois at Chicago, located just west of downtown Chicago. The spectrometer, together with a cryoprobe-equipped 800MHz spectrometer that will be installed next year, will be operated as a shared resource for all NIH-funded Chicago area investigators working on biomedically important problems. This shared resource will permit a complete transformation of protein NMR work in Chicago, from isolated campus-based investigations at relatively low field, to an interactive community of top rate investigators with access to the best equipment available. Such synergy is also likely to have a positive effect on bringing about other inter-university collaborations between Chicago universities that have previously had a more competitive than collaborative relationship. An immediate example, that also illustrates the way in which this resource will serve the current and future national scientific needs, is the development of a UIC-University of Chicago-Northwestern Research Center of Excellence in bioterrorism-related research. NMR, including the resource described here, will play a key role in this Center and in related research. As part of the facility, a resource will be established for development of procedures for intein-based segmental isotopic labeling of proteins, as well as for production of segmentally labeled DNA and RNA species.

描述（由申请人提供）：伊利诺伊大学芝加哥分校（UIC）与芝加哥和芝加哥地区其他主要研究机构，即西北大学、芝加哥大学、洛约拉大学和威斯康星医学院合作，寻求资金用于购买窄孔 21Tesla (900MHz) NMR 波谱仪和冷冻探针，用于在溶液中对具有生物医学重要性的蛋白质和蛋白质复合物进行高分辨率研究，这些研究需要使用最高的可用磁场 力量。该光谱仪将安装在伊利诺伊大学芝加哥分校医学中心校园内一座专门建造的、新建的独立结构生物学仪器大楼内，该大楼位于芝加哥市中心以西。该光谱仪与将于明年安装的配备冷冻探针的 800MHz 光谱仪一起，将作为所有 NIH 资助的芝加哥地区研究人员研究生物医学重要问题的共享资源。这种共享资源将使芝加哥的蛋白质核磁共振工作实现彻底转变，从相对较低领域的孤立校园研究，转变为由顶级研究人员组成的互动社区，可以使用最好的设备。这种协同作用也可能对芝加哥大学之间的其他大学间合作产生积极影响，这些大学之间以前的竞争关系大于合作关系。一个直接的例子是伊利诺伊大学芝加哥分校-西北生物恐怖主义相关研究卓越研究中心的发展，这也说明了该资源将如何服务于当前和未来的国家科学需求。 NMR，包括此处描述的资源，将在该中心和相关研究中发挥关键作用。作为该设施的一部分，将建立一个资源，用于开发基于内含肽的片段同位素标记蛋白质的程序，以及用于生产片段标记的 DNA 和 RNA 物种。