LEUKOTOXIN DIOL FROM CORN COB ANIMAL BEDDING STIMULATES BREAST CANCER CELLS

玉米芯动物垫料中的白细胞毒素二醇可刺激乳腺癌细胞

• 批准号: 7355163
• 负责人: BARRY Matthew MARKAVERICH
• 金额: $ 0.59万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7355163
• 关键词: LEUKOTOXIN; DIOL; CORN; COB; ANIMAL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Ground corncob contains a number of mitogenic agents that stimulate breast and prostate cancer cell proliferation in vitro and in vivo and also disrupt male and female reproductive function and cyclicity. Purification of ground corncob bedding extracts by HPLC revealed a major peak of mitogentic activity eluting from a C8 reversed phase column that contained 9, 10-dihydroxy-12-octadecenoicacid (LTX-diol), a well-known leukotoxin-diol. This mixture of the two positional isomers (LTX-diols) stimulated the proliferation of MCF-7 human breast cancer cells in vitro at concentrations ranging from 13-26 uM. Thus, LTX-diol derived from exogenous or endogenous sources or pathways may promote or enhance malignant cell proliferation that we suspect are endocrine.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。磨碎的玉米芯含有多种促有丝分裂剂，可在体外和体内刺激乳腺癌和前列腺癌细胞增殖，并破坏男性和女性的生殖功能和周期性。通过 HPLC 纯化磨碎的玉米芯垫料提取物，发现从含有 9, 10-二羟基-12-十八烯酸 (LTX-二醇)（一种众所周知的白细胞毒素二醇）的 C8 反相柱中洗脱出促有丝分裂活性的主峰。这种两种位置异构体（LTX-二醇）的混合物在浓度范围为 13-26 uM 时可在体外刺激 MCF-7 人乳腺癌细胞的增殖。因此，源自外源或内源来源或途径的LTX-二醇可能促进或增强我们怀疑是内分泌的恶性细胞增殖。