CDOn: A Novel Marker of Neuronal Stem Cells

CDOn：神经元干细胞的新型标记物

基本信息

批准号：
7647581
负责人：
Lawrence D Recht
金额：
$ 4万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2010-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7647581
关键词：
Abbreviations Address Adult Affect Alkaline Phosphatase Alleles Animals Ara-C B-Lymphocytes Biological Assay Blast Cell Brain Bromodeoxyuridine Cataloging Catalogs Cell Cycle Cell Surface Receptors Cells Cellular biology Clonality Communities Cues Cultured Cells Cytotoxic agent Data Development Disease Electrons Environment Experimental Designs Exposure to Galactosidase Genes Genetic Transcription Glial Fibrillary Acidic Protein Grant Green Fluorescent Proteins Holoprosencephaly Human Immunohistochemistry In Situ Hybridization In Vitro Injury LacZ Genes Lead Maintenance Methods Microforms Molecular Profiling Mus Mutation Natural regeneration Neoplasms Neuraxis Neurobiology Neuroglia Neurons Numbers Organ Pattern Physiologic pulse Play Population Principal Investigator Proliferating Proliferation Marker Property Proteins Pulse taking Purpose Radial Reagent Recruitment Activity Reporter Reporting Role SHH gene Signal Transduction Staging Stem Cell Research Stem cells Terminology Tissues Transplantation Undifferentiated Ventricular adult stem cell base cell type daughter cell gene function in vivo insight interest multipotent cell mutant nerve stem cell nervous system disorder novel nucleotide analog null mutation phospholipase A2-activating protein polysialyl neural cell adhesion molecule progenitor programs promoter receptor receptor expression reconstitution relating to nervous system research study sonic hedgehog receptor transmission process

项目摘要

DESCRIPTION (provided by applicant): Stem cell activity has been reported in various adult tissues and organs, including some discrete regions within the adult brain. The identification of a pool of cells within the mammalian CNS with the properties both to self-renew as well as differentiate into all neural cell types has generated much excitement in the neurobiology community because of its relevance to brain regeneration, injury and neoplasia. Our ability to harness the regenerative capacity of stem cells to reduce the burden of neurological disease and damage hinges on understanding the fundamental biology of these cells. Making progress in adult stem cell research is dependent on clearly identifying and isolating discrete cell populations, characterizing these cells, as well as on deciphering signals in the surrounding environment that instruct these cells whether to remain as stem cells or to differentiate. We previously isolated a cell surface receptor, CDOn, whose expression pattern strikingly coincides with regions in the adult central nervous system associated with stem cell activity. Interestingly, the loss of this gene function results in a microform of holoprosencephaly. The purpose of the experiments proposed here are to explore the possibility that CDOn receptor expression delineates the sup-population of slowly cycling stem cells in the adult brain. We plan to establish the identity of the cells in the stem cell niche that express CDOn and to substantiate the potential usefulness of CDOn expression as a marker for specifically isolating these cells in vitro and in vivo. Because CDOn is a receptor, we believe that revealing the identity of cells in the stem cell niche that express the CDOn receptor in comparison to cells that do not will yield insights into how cells respond differently to extrinsic cues within the microenvironment of the stem cell niche.

描述（由申请人提供）：已在各种成人组织和器官中报道了干细胞活性，包括成人大脑内的某些离散区域。鉴定具有自我更新和分化为所有神经细胞类型的哺乳动物中枢神经系统中的细胞池在神经生物学群落中引起了极大的兴奋，因为它与脑再生，损伤和肿瘤相关。我们利用干细胞再生能力减轻神经系统疾病负担和损害的能力取决于了解这些细胞的基本生物学。在成年干细胞研究中取得进展取决于明确识别和隔离离散细胞群体，表征这些细胞，以及在周围环境中解密的信号，这些信号指示这些细胞保持为干细胞或分化。我们先前分离了细胞表面受体CDON，其表达模式与与干细胞活性相关的成年中枢神经系统中的区域显着相吻合。有趣的是，该基因功能的丧失会导致单脑脑的微型细菌。这里提出的实验的目的是探讨CDON受体表达描述成人大脑中缓慢循环干细胞的SUP人口的可能性。我们计划建立表达CDON的干细胞生态位细胞的身份，并证实CDON表达作为特异性分离这些细胞体外和体内特异性分离的标记的潜在实用性。因为CDON是一种受体，我们认为与未能对细胞在干细胞生态环境中微观环境内的外在线索的反应不同的细胞相比，揭示了表达CDON受体的细胞中的细胞的身份，这些受体表达了CDON受体。。