COBRE: UKS: CORE B: HIGH THROUGHPUT SCREENING & TARGET IDENTIFICATION

COBRE：UKS：核心 B：高通量筛选

• 批准号: 7609705
• 负责人: ERNST SCHONBRUNN
• 金额: $ 21.38万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2008-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7609705
• 关键词: Biochemical; Biological; Biological Assay; Biomedical Research; Cell physiology; Cells; Centers of Research Excellence; Communities; Computer Retrieval of Information on Scientific Projects Database; Disease; Evaluation; Funding; Grant; Human Resources; Individual; Institution; Kansas; Laboratories; Numbers; Operative Surgical Procedures; Physiological; Proteins; Purpose; Research; Research Personnel; Resources; Screening procedure; Services; Source; United States National Institutes of Health; Universities; Work; analog; assay development; base; design; drug discovery; high throughput screening; interest; small molecule; small molecule libraries; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. High throughput screening (HTS) has become a powerful tool for biomedical research and a resource for drug discovery. The purpose of HTS is to screen large numbers of small molecules that interact selectively with biological targets so that they may be used as probes to understand the function of newly characterized proteins and to analyze physiological e processes, cellular phenomena, and disease mechanisms. The HTS laboratory at the University of Kansas (www.hts.ku.edu) was established through the COBRE grant and has provided service to COBRE investigators, and the local and national research community. We have supported and will continue to support COBRE investigators and will also expand both the scope and capacity of our high throughput screening to meet the increased needs of biomedical researchers. Our objectives are (1) to strengthen our assay development capacity to import and adapt biochemical and cell-based assays to HTS format and (2) to strengthen our high throughput screening capacity to carry out efficient screening campaigns. We work with individual research investigators to adapt their bench-top assays to suit HTS operation and use these successfully adapted HTS assays to screen our existing chemical library of small organic compounds for molecules showing biological activity. Because of the existing HTS facility and personnel at the University of Kansas, we are certain that further expansion will make significant contributions toward the discovery of new interesting compounds and toward the design, synthesis and evaluation of analogues derived from original hits coming from HTS.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 高通量筛选（HTS）已成为生物医学研究的强大工具和药物发现的资源。 HTS的目的是筛选大量与生物靶标选择性相互作用的小分子，以便将它们用作探针来了解新表征的蛋白质的功能并分析生理过程、细胞现象和疾病机制。堪萨斯大学的 HTS 实验室 (www.hts.ku.edu) 是通过 COBRE 拨款建立的，为 COBRE 研究人员以及当地和国家研究界提供服务。我们已经并将继续支持 COBRE 研究人员，还将扩大我们高通量筛选的范围和能力，以满足生物医学研究人员日益增长的需求。我们的目标是 (1) 加强我们的检测开发能力，以导入生化和细胞检测并使其适应 HTS 格式；(2) 加强我们的高通量筛选能力，以开展高效的筛选活动。我们与个别研究人员合作，调整他们的台式测定以适应 HTS 操作，并使用这些成功调整的 HTS 测定来筛选我们现有的小有机化合物化学库中显示生物活性的分子。由于堪萨斯大学现有的 HTS 设施和人员，我们确信进一步的扩展将为发现新的有趣化合物以及从 HTS 的原始命中衍生的类似物的设计、合成和评估做出重大贡献。