Effect of exercise and weight loss on adipose tissue biology

运动和减肥对脂肪组织生物学的影响

• 批准号: 7359458
• 负责人: CORNELIA M ULRICH
• 金额: $ 19.8万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-01 至 2010-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7359458
• 关键词: Abdomen; Adipose tissue; Androgens; Aromatase; Biological Factors; Biological Markers; Biology; Body Composition; Body Weight; Body Weight Changes; Body Weight decreased; Body fat; Body mass index; Caloric Restriction; Calories; Clinical Trials; Colorectal; Data; Diet; Exercise; Fatty acid glycerol esters; Funding; Gene Chips; Gene Expression; Gonadal Steroid Hormones; Hour; Human; Inflammatory; Insulin; Insulin Resistance; Knowledge; Leptin; Life Style; Link; Malignant Neoplasms; Mediating; Messenger RNA; Obesity; Overweight; Participant; Pathway interactions; Pattern; Physical activity; Play; Postmenopause; Production; Randomized Controlled Trials; Recommendation; Risk; Role; Somatomedins; Testing; Thinking; Tissue Sample; Tissues; Week; Woman; adipokines; adiponectin; base; cancer risk; cancer type; carcinogenesis; cytokine; diet and exercise; dietary restriction; energy balance; lifestyle intervention; lipid metabolism; mRNA Expression; malignant breast neoplasm; protein expression; sedentary; steroid hormone; subcutaneous; Abdomen; Adipose tissue; Androgens; Aromatase; Biological Factors; Biological Markers; Biology; Body Composition; Body Weight; Body Weight Changes; Body Weight decreased; Body fat; Body mass index; Caloric Restriction; Calories; Clinical Trials; Colorectal; Data; Diet; Exercise; Fatty acid glycerol esters; Funding; Gene Chips; Gene Expression; Gonadal Steroid Hormones; Hour; Human; Inflammatory; Insulin; Insulin Resistance; Knowledge; Leptin; Life Style; Link; Malignant Neoplasms; Mediating; Messenger RNA; Obesity; Overweight; Participant; Pathway interactions; Pattern; Physical activity; Play; Postmenopause; Production; Randomized Controlled Trials; Recommendation; Risk; Role; Somatomedins; Testing; Thinking; Tissue Sample; Tissues; Week; Woman; adipokines; adiponectin; base; cancer risk; cancer type; carcinogenesis; cytokine; diet and exercise; dietary restriction; energy balance; lifestyle intervention; lipid metabolism; mRNA Expression; malignant breast neoplasm; protein expression; sedentary; steroid hormone; subcutaneous

DESCRIPTION (provided by applicant): Higher levels of physical activity and lower body weight or body fat are associated with a decreased risk of several types of cancer, in particular colorectal and postmenopausal breast cancer. Possible mechanistic pathways for this association involve inflammatory factors, steroid hormones, insulin like growth factors or insulin resistance, and lipid metabolism. Many of these mechanisms can be mediated by changes in energy balance such as weight change or changes in body composition. Adipose tissue plays a role in several of the proposed mechanisms, particularly via the production of proinflammatory cytokines and sex hormones. The proposed project will examine the intersection between energy balance specifically diet, physical activity and weight change and adipose tissue biology.There will be ancillary to a funded human clinical trial of exercise and caloric restriction in overweight or obese, previously sedentary, postmenopausal women. The project will collect subcutaneous abdominal adipose tissue sample sat base line and at 6 months in 60 women. Specific aims are (1) to investigate the effects of exercise (n=15), reduced calorie diet (n=15), exercise+diet (n=15) and usual lifestyle control (n=15) on adipose tissue mRNA and protein expression of specific proinflammatory cytokines (i.e.IL6andTNF1) and adipokines(i.e. leptin and adiponectin), as well as mRNA expression related to sex steroid hormone production (i.e. aromatase); (2) to investigate the impact of changes in body weight (kg) or body mass index (kg/m2) and physical activity (METhoursweek) on the above biomarkers ; and (3) to explore changes in adipose tissue gene expression patterns using the Affymetrix U133 plus 2.0 Gene Chip microarray ,focusing on a subset of participants in the exercise + diet group (n=10) versus controls (n=10). The exercise + diet group is expected to have the largest change in percent body fat, thus providing the strongest contrast. To our knowledge, this study will be the first randomized controlled trial to test the effects of exercise, both alone and in combination with a calorie restricted diet, on mRNA and protein expression in subcutaneous adipose tissue in humans. Energy balance is becoming increasingly recognized as an important factor in cancer risk. The adipocyteis at the interface of the association between energy balance and the hypothesized mechanisms linking energy balance to carcinogenesis. Examination of adipose tissue is critical to further the understanding of this link. This trial will provide data on the effect of a lifestyle intervention on important aspects of adipose tissue biology. PROJECT NARRATIVE This project will investigate the effects of diet and exercise on fat tissue. Being physically inactive and overweight or obese has been linked to increased risk of some cancers. Thus, we want to understand how physical activity, a diet low in calories, and body composition changes alter biological factors in fat tissue that are thought to be associated with cancer risk. This information will allow us to develop better lifestyle recommendations on how to reduce cancer risk.

描述（由申请人提供）：较高水平的体育活动和较低的体重或体内脂肪与几种类型的癌症（尤其是结直肠癌和绝经后乳腺癌）的风险降低有关。该关联的可能机械途径涉及炎症因子，类固醇激素，胰岛素（例如生长因子或胰岛素抵抗）以及脂质代谢。这些机制中的许多可以通过能量平衡的变化（例如体重变化或人体组成变化）来介导。脂肪组织在几种提出的机制中起作用，尤其是通过产生促炎细胞因子和性激素。拟议的项目将研究能量平衡，特定于饮食，体育活动和体重变化与脂肪组织生物学之间的交集。在超重或肥胖的，以前久坐的绝经后妇女中，将有辅助人类对运动和热量限制的人类运动和热量限制临床试验。该项目将收集皮下腹部脂肪组织样品SAT基线，并在60名女性中收集6个月的6个月。具体目的是（1）研究运动（n = 15），降低卡路里饮食（n = 15），运动+饮食（n = 15）和通常的生活方式控制（n = 15）对脂肪组织mRNA和蛋白质表达的特定促炎细胞因子的蛋白质表达（即与脂肪素（即脂肪素）相关）（即，脂蛋白）和脂肪素。激素产生（即芳香酶）； （2）研究体重变化（kg）或体重指数（kg/m2）和体育活动（甲基周刊）对上述生物标志物的影响； （3）使用Affymetrix U133加2.0基因芯片微阵列探索脂肪组织基因表达模式的变化，重点介绍了运动 +饮食组（n = 10）的一部分与对照组（n = 10）。锻炼 +饮食组预计将具有体内脂肪百分比的最大变化，从而提供最强的对比度。据我们所知，这项研究将是第一个随机对照试验，即单独和与卡路里限制饮食，对人类皮下脂肪组织中的mRNA和蛋白质表达结合进行运动的影响。 能量平衡越来越被公认为是癌症风险的重要因素。能量平衡与能量平衡与癌变的假设机制之间关联界面处的脂肪细胞。脂肪组织的检查对于进一步理解这种联系至关重要。该试验将提供有关生活方式干预对脂肪组织生物学重要方面的影响的数据。项目叙述该项目将研究饮食和运动对脂肪组织的影响。身体不活跃和超重或肥胖与某些癌症的风险增加有关。因此，我们想了解体育锻炼，饮食低的卡路里和身体成分如何改变脂肪组织中的生物学因素，这些因素被认为与癌症风险有关。这些信息将使我们能够为如何降低癌症风险提出更好的生活方式建议。