GENETIC & METABOLIC ADAPTATION BY SALMONELLA TO THE NATURAL ENVIRONMENT

基因

• 批准号: 7381775
• 负责人: HAZEL A BARTON
• 金额: $ 11.55万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381775
• 关键词: GENETIC; METABOLIC; ADAPTATION; SALMONELLA; NATURAL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Salmonella is generally considered a low-frequency commensal and low-grade pathogen with very limited ability to survive long term in the environment outside of a host organism. Recent work has identified a constellation of interactive metabolic functions shared by all Salmonellae, but absent from its nearest relative E. coli. The constellation of Salmonella-specific functions includes de novo synthesis of cobalamin (B12), use of B12 in degradation of ethanolamine and propanediol, anaerobic respiration of tetrathionate to thiosulfate (ttr genes), and subsequent reduction to sulfite (phs genes) and sulfide (asr genes). All these functions would be used when Salmonella grows anaerobically on ethanolamine or propanediol in the presence of reducible sulfur compounds. Under these conditions, Salmonella forms granules of mineral sulfur, which it (but not E. coli) can later reduce by a process that requires the phs genes. While these functions may be important to pathogenesis, we think it more likely that they suggest a significant lifestyle for Salmonella in particular soil environments. Our work has been geared to understanding whether there is an environmental niche for Salmonella and the potential impact that such an ecological niche will have on our understanding of the pathogenesis of this organism.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。通常认为沙门氏菌被认为是低频共生和低级病原体，其在宿主生物体以外的环境中长期生存的能力非常有限。最近的工作已经确定了所有沙门氏菌共有的交互式代谢功能的星座，但没有其最近的相对大肠杆菌。沙门氏菌特异性功能的星座包括从头合成钴胺素（B12），使用B12在乙醇胺降解和丙二醇的降解，四立酸酯向硫代硫酸盐呼吸至硫代硫酸盐（TTR基因），以及随后还原至Sulfite（Sulfite（Sulfite）（PHS基因）和Sules基因（ASR基因）。当沙门氏菌在乙醇胺或丙二醇的存在下，在可还原硫化合物存在下，将使用所有这些功能。在这些条件下，沙门氏菌形成矿物硫的颗粒，它（但不是大肠杆菌）可以通过需要pHS基因的过程来减少。尽管这些功能可能对发病机理很重要，但我们认为它们更有可能在特定土壤环境中为沙门氏菌提出重要的生活方式。我们的工作旨在了解沙门氏菌是否有环境利基市场，以及这种生态位对我们对这种生物体发病机理的理解的潜在影响。