IN VIVO AND IN VITRO EFFECTS OF THE PESTICIDE ATRAZINE ON BASAL GANGLIA FUNCTION

农药阿特拉津对基底节功能的体内外影响

• 批准号: 7381806
• 负责人: NIKOLAY M FILIPOV
• 金额: $ 8.83万
• 依托单位: MISSISSIPPI STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381806
• 关键词: VIVO; VITRO; EFFECTS; PESTICIDE; ATRAZINE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Parkinson¿s Disease (PD) is a debilitating neurodegenerative disease of the basal ganglia and has an elusive etiology. Recently, a possible link between PD and pesticide exposure has been suggested. The herbicide atrazine is the most widely used pesticide in the US, with ground water contamination being a major concern. Currently, there are no data addressing atrazine effects on basal ganglia function. This research project explores the hypothesis that short-term exposure to atrazine would result in persistent neurotoxicity with the degree of neurotoxicity being dependent on the age of the subjects. Moreover, it is hypothesized that chronic, low-level exposure to atrazine will not be neurotoxic on its own, but it will potentiate the toxicity of a typical basal ganglia neurotoxicant such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). To address this hypothesis, C57BL/6 mice, 1, 3, and 10 months of age, will be exposed to various dosages of atrazine for either short-term (14 days) or long-term (up to 26 weeks) and the degree of dopaminergic neurodegeneration will be assessed after termination of atrazine exposure. Additionally, to gain a mechanistic insight on atrazine-induced basal ganglia neurotoxicity, in vitro experiments with striatal slices and mixed mesencephalic cultures will be performed.

该主题是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是针对该中心的，这不是调查人员的机构。帕金森病（PD）是一种令人衰弱的基础神经节神经退行性疾病，具有难以捉摸的病因。最近，已经提出了PD与农药暴露之间的可能联系。除草剂阿雷津是美国使用最广泛的农药，地下水污染是一个主要问题。当前，没有数据解决阿atrazine对基底神经节功能的影响。该研究项目探讨了以下假设：短期暴露于阿特拉津会导致持续的神经毒性，而神经毒性的程度取决于受试者的年龄。此外，假设慢性，低水平暴露于阿特拉嗪不会单独是神经毒性，但它可能会产生典型的基底神经节神经毒性剂的毒性，例如1-甲基-4-苯基-4-苯基-1,2,3,6,6-6-甲基二羟基吡啶（MPTP）。为了解决这一假设，C57BL/6小鼠，1、3和10个月。年龄将在短期（14天）或长期（长达26周）中暴露于各种剂量的阿特拉津，并且在终止阿特拉津暴露后，将评估多巴胺能神经变性的程度。此外，为了获得有关阿atrazine诱导的低音神经毒性的机理见解，将进行纹状体切片和混合且脑培养物的体外实验。