ROLE OF THE ANTIZYME FAMILY DURING XENOPUS DEVELOPMENT

抗酶家族在非洲爪蟾发育过程中的作用

• 批准号: 7381352
• 负责人: Charles Richard Toth
• 金额: $ 5.84万
• 依托单位: UNIVERSITY OF RHODE ISLAND
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381352
• 关键词: ROLE; ANTIZYME; FAMILY; DURING; XENOPUS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Polyamines are important organic cations that are essential for life. Cells that are actively proliferating such as embryonic cells or cancer cells, have strict needs for polyamines. Thus, manipulation of polyamine levels can have profound effects on growth. Cells maintain well-regulated pathways that control polyamine levels at optimum levels for cell growth. Antizyme (AZ) is a crucial protein that influences the rate of cell proliferation by controlling the levels of polyamines. An aim of this project is to characterize the regulation of polyamine levels and effects on cell growth using a developmental model of proliferation, Xenopus laevis embryogenesis. A focus of the work will be to determine how AZ regulates the proliferative capacity of Xenopus embryonic cells. There has not been a systematic study of antizyme function using a developmental model system such as the amphibian Xenopus laevis. This project will characterize the expression pattern of the AZ gene family using quantitative RT-PCR, Northerns, Westerns, and two dimensional protein gels during the early development of Xenopus embryos. The function of the AZ family will be studied by microinjection experiments that will force AZ expression at the one cell embryo stage. This will allow for a study of whether AZ can dominantly control cell proliferation. Thus, the outcome of this project will be the elucidation of the role of the AZ family in proliferation and development using a tractable organism, Xenopus laevis. Since the developmental pathways of Xenopus laevis are closely related to the same mammalian pathways, this project will help to shed light on the role of AZ in mice and humans with potential applications to the growth of cancer cells.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。多胺是重要的有机阳离子，对生命至关重要。积极增殖的细胞（例如胚胎细胞或癌细胞）对多胺有严格的需求。因此，对多胺水平的操纵可以对生长产生深远的影响。细胞保持良好的调节途径，以控制多胺水平以最佳水平以用于细胞生长。抗酶（AZ）是一种至关重要的蛋白质，通过控制多胺水平来影响细胞增殖速率。 An aim of this project is to characterize the regulation of polyamine levels and effects on cell growth using a developmental model of proliferation, Xenopus laevis embryogenesis.这项工作的重点是确定AZ如何调节异爪蟾细胞的增殖能力。使用诸如两栖动物Xenopus laevis等发展模型系统的抗酶功能的系统研究。 This project will characterize the expression pattern of the AZ gene family using quantitative RT-PCR, Northerns, Westerns, and two dimensional protein gels during the early development of Xenopus embryos. AZ家族的功能将通过显微注射实验研究，该实验将迫使AZ表达在一个细胞胚胎阶段。这将允许研究AZ是否可以主要控制细胞增殖。 Thus, the outcome of this project will be the elucidation of the role of the AZ family in proliferation and development using a tractable organism, Xenopus laevis. Since the developmental pathways of Xenopus laevis are closely related to the same mammalian pathways, this project will help to shed light on the role of AZ in mice and humans with potential applications to the growth of cancer cells.