CHARACTERIZATION OF THE DISRUPTED LOCUS IN A TOXOPLASMA GONDII BRADYZOITE DIFFER

弓形虫缓殖子中被破坏位点的特征不同

• 批准号: 7381423
• 负责人: MARIANA L MATRAJT
• 金额: $ 1.69万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7381423
• 关键词: CHARACTERIZATION; DISRUPTED; LOCUS; TOXOPLASMA; GONDII

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The human pathogen Toxoplasma gondii is one of the most widely distributed protozoan parasites, infecting approximately one-third of the world?s population. Two forms characterize asexual reproduction of T. gondii; rapidly growing ?tachyzoites? and latent ?bradyzoite? tissue cysts. These two developmental stages are central to disease propagation and causation. The interconversion between tachyzoites and bradyzoites, at the heart of parasite survival and pathogenicity, is poorly understood at a molecular and genetic level, which makes understanding this process an important goal. Recently, a genetic screen was developed to identify regulatory genes that control parasite differentiation and mutants that fail to convert to bradyzoites under differentiation conditions have been isolated. The disrupted locus in one of these mutants (mutant B7) has been identified and the expression of a developmentally regulated transcript (B41) has been abolished in the mutant parasites. B41 is transcribed; yet it contains no obvious open reading frame. The goal of this project is to functionally characterize the disrupted locus in the mutant B7 and test the hypothesis that this locus codes for a functional non-coding RNA that plays a critical role in bradyzoite formation.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。人类病原体弓形虫弓形虫是分布最广泛的原生动物寄生虫之一，感染了世界上约三分之一的人群。两种形式表征了T. gondii的无性繁殖；快速生长？tachyzoites？和潜在的？组织囊肿。这两个发展阶段对于疾病的传播和因果关系至关重要。在寄生虫生存和致病性的核心，速氮和胸肌之间的相互转换在分子和遗传水平上是很少了解的，这使得理解这一过程是重要的目标。最近，已经开发了一个遗传筛选，以鉴定控制寄生虫分化的调节基因和在分化条件下未能转化为铁二氮化物的突变体。这些突变体之一（突变体B7）中的破坏基因座已经被鉴定出来，并且在突变寄生虫中废除了发育调节的转录本（B41）的表达。 B41被转录；但是它没有明显的开放阅读框架。该项目的目的是在功能上表征突变体B7中的中断基因座，并测试该基因座代码为功能性非编码RNA的假设，在Bradyzoite形成中起着至关重要的作用。