A state of the art multiparametric flow cytometry analysis system for multidisciplinary stem cell research

用于多学科干细胞研究的最先进的多参数流式细胞术分析系统

• 批准号: BB/E012841/1
• 负责人: Majlinda Lako
• 金额: $ 32.05万
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2007
• 资助国家: 英国
• 起止时间: 2007 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FE012841%2F1
• 关键词: state; art; multiparametric; flow; cytometry

Stem cells are capable of essentially extensive growth whilst retaining the ability to differentiate into most of the cell types found in the adult but unless we can find ways to grow them in large numbers while directing them to differentiate into therapeutically useful cells much of this potential will not be realised. A major obstacle impacting the derivation of differentiated cell types from stem cells is that current in vitro differentiation strategies often only produced the desired cells in small numbers. This means they must be separated from the other cell types present and one of the best techniques we have for doing this is flow cytometry. This technology relies upon the binding of fluorescent labelled antibodies to the surfaces of our desired cells such that when they pass through the flow cytometer they can be separated from the others and retain their viability. If appropriate antibodies are available, up to 12 different colours can be analysed at any one time using the instrumentation detailed in this proposal and this offers us an unparalleled advantage in cell identification and separation. There are a number of active research groups at the Institute of Human Genetics investigating a range of differentiation protocols designed to produce useful cells from a range of stem cells and flow cytometry instrumentation is not only indispensable to this work but is also subject to very heavy demand. In view of this, a successful outcome of this proposal would greatly enhance our ability to develop useful techniques for stem cell differentiation that will ultimately benefit medical science in general.

干细胞能够基本上具有广泛的生长，同时保留了分化为成年人中大多数细胞类型的能力，但是除非我们能够找到大量生长的方法，同时却指示它们分化为治疗有用的细胞，这将无法实现。影响干细胞分化细胞类型的衍生细胞类型的主要障碍是，当前的体外分化策略通常仅在少量产生所需的细胞。这意味着它们必须与存在的其他细胞类型分开，而我们为此做的最好的技术之一就是流式细胞仪。该技术依赖于荧光标有抗体与我们所需细胞的表面的结合，因此当它们通过流式细胞仪时，它们可以与其他细胞分离并保留其生存能力。如果可以使用适当的抗体，则使用此提案中详细介绍的仪器，最多可以分析多达12种不同的颜色，这为我们提供了无与伦比的细胞识别和分离优势。人类遗传学研究所研究了许多活跃的研究小组，研究了一系列分化方案，旨在从一系列干细胞中产生有用的细胞，流式细胞仪仪器不仅在这项工作中是必不可少的，而且需求非常大。鉴于这一点，该提案的成功结果将大大增强我们开发有用的干细胞分化技术的能力，最终将使医学科学受益。

项目成果

Brief Report: Inhibition of miR-145 Enhances Reprogramming of Human Dermal Fibroblasts to Induced Pluripotent Stem Cells.

• DOI: 10.1002/stem.2220
• 发表时间: 2016-01
• 期刊: Stem cells (Dayton, Ohio)
• 作者: Barta T;Peskova L;Collin J;Montaner D;Neganova I;Armstrong L;Lako M
• 通讯作者: Lako M

Concise Review: Getting to the Core of Inherited Bone Marrow Failures.

• DOI: 10.1002/stem.2543
• 发表时间: 2017-02
• 期刊: Stem cells (Dayton, Ohio)
• 作者: Adam S;Melguizo Sanchis D;El-Kamah G;Samarasinghe S;Alharthi S;Armstrong L;Lako M
• 通讯作者: Lako M

Concise Review: The Epigenetic Contribution to Stem Cell Ageing: Can We Rejuvenate Our Older Cells?

• DOI: 10.1002/stem.1720
• 发表时间: 2014-09-01
• 期刊: STEM CELLS
• 影响因子: 5.2
• 作者: Armstrong, Lyle;Al-Aama, Jumana;Lako, Majlinda
• 通讯作者: Lako, Majlinda