Retinoic acid in motor neuron specification

运动神经元规范中的视黄酸

• 批准号: 7029763
• 负责人: EDUARDO ROSA-MOLINAR
• 金额: $ 31.49万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-08 至 2009-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7029763
• 关键词: Puerto Rico; alternatives to animals in research; cell biology; cell cell interaction; cell differentiation; cell migration; cerebral ventricles; cooperative study; developmental neurobiology; electroporation; enzyme activity; fish; glia; immunocytochemistry; minority institution research support; molecular /cellular imaging; molecular probes; motor neurons; neurogenesis; nonmammalian vertebrate embryology; retinoate; spinal cord; vitamin metabolism; zebrafish

The proposed project of Drs. Eduardo Rosa-Molinar, the University of Puerto Rico-Rio Piedras (UPR-RP), and Scott E. Fraser of the California Institute of Technology (CalTech) will result in a greater understanding of the cellular interactions that guide the development of the spinal cord. In particular, the experiments will establish the phenotypes of the cells that contain the enzymes synthesizing and degrading retinoic acid (RA) and elucidate their role in guiding the genesis, migration, and fate of germinal cells in the ependyma of the embryonic spinal cord, specifically, secondary motor neurons. The experimental plan builds upon previous National Science Foundation funded work performed in the Rosa-Molinar laboratory. That work established both the RA metabolism and the functional neuroanatomy of the spinal cord in the developing and mature Western Mosquitofish, Gambusia affinis affinis (hereafter "mosquitofish"). The proposed project's test experiments on the interactions that guide the development and differentiation of the spinal cord will take advantage of some of the unique attributes of the mosquitofish. As described in detail elsewhere in this grant application, complementary work will be performed in the two laboratories.

博士提出的项目。波多黎各里约彼德拉斯大学 (UPR-RP) 的 Eduardo Rosa-Molinar 和加州理工学院 (CalTech) 的 Scott E. Fraser 将更好地了解指导脊柱发育的细胞相互作用绳索。特别是，这些实验将建立含有合成和降解视黄酸（RA）的酶的细胞的表型，并阐明它们在指导胚胎脊髓室管膜中生发细胞的发生、迁移和命运中的作用，特别是，次级运动神经元。该实验计划建立在之前国家科学基金会资助的罗莎-莫利纳尔实验室进行的工作的基础上。这项工作建立了发育和成熟的西方食蚊鱼 Gambusia affinis affinis（以下简称“食蚊鱼”）的 RA 代谢和脊髓的功能性神经解剖学。该项目针对指导脊髓发育和分化的相互作用进行的测试实验将利用蚊鱼的一些独特属性。正如本拨款申请中其他部分详细描述的，两个实验室将进行补充工作。