Microbiology Core

微生物学核心

• 批准号: 7250822
• 负责人: PATRICK MARTIN
• 金额: $ 26.74万
• 依托单位: OSEL, INC.
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7250822
• 关键词: HIV infections; Lactobacillus; antiAIDS agent; biomedical facility; biotechnology; biotherapeutic agent; communicable disease control; communicable disease transmission; cooperative study; drug design /synthesis /production; drug vehicle; fermentation; genetic manipulation; genetic strain; method development; microbiology; topical drug application

The Microbiology core will be used broadly by all three of the individual project teams. The efficient operations of this central core will be fundamental to the success of this Program Project. The overall Program Project is directed at applying vaginally derived lactobacilli to introduce anti-HIV protein microbicides to mucosal sites within the vagina or rectum as a means of preventing HIV transmission. The Microbiology core has three main objectives. Its first goal is to maintain a library of well characterized vaginal lactobacilli and to continue development of advanced methods to improve genetic manipulation of these microorganisms. These strains and genetic methods will be made available to the various laboratories participating in this Program Project. In particular, this core will undertake an effort to cure a preferred strain of a vaginal Lactobacillus of a resident bacteriophage. In addition, this facitity will pursue the genetic manipulation of a Lactobacillus species that appears to be the dominant colonizer of the vaginal flora of the macaque and humans. While prior attempts to manipulate this species af Lactobacillus have proven difficult, successful engineering of this strain could lead to improved products for testing in the macaque and eventual human clinical use. A second goal, is to refine and optimize processes already operational at Osel for the manufacturing and formulating Lactobacilli. It is desirable to produce formulations of these bacteria that are relatively stable in harsh environments, thus affording widespread use of these lactobacillus-based products. Finally, the Microbiology core will provide encapsulated formulations of genetically modified lactobacilli in support of proposed primate safety, colonization, and efficacy studies, as well as perform a detailed microbiological analysis of samples derived from animals that have received these products.

微生物核心将由所有三个项目团队广泛使用。该中心核心的有效操作将是该计划项目成功的基础。整个程序项目旨在施用阴道衍生的乳酸杆菌，以将抗HIV蛋白杀菌剂引入阴道或直肠内的粘膜部位，以防止HIV传播。 微生物核心具有三个主要目标。它的第一个目标是维持具有良好特征的阴道乳酸杆菌的库，并继续开发高级方法以改善对这些微生物的遗传操纵。这些菌株和遗传方法将提供给参与该计划项目的各种实验室。特别是，该核心将努力治愈居民噬菌体的阴道乳杆菌的首选菌株。此外，这种面孔将追求对乳杆菌物种的遗传操纵，该乳杆菌似乎是猕猴和人类阴道菌群的主要结肠化合物。事先尝试操纵该物种Af lactobacillus被证明很困难，但 这种菌株的成功工程可能会导致改进的产品用于在猕猴和最终人类临床使用中进行测试。第二个目标是完善和优化已经在OSEL上运行的过程，用于制造和制造乳杆菌。希望产生这些细菌的配方，这些细菌在恶劣的环境中相对稳定，从而可以广泛使用这些基于乳杆菌的产品。 最后，微生物核心将提供封装的遗传改性乳酸乳杆菌的配方，以支持提出的灵长类动物安全，定殖和疗效研究，并对从已收到这些产物的动物中得出的样品进行详细的微生物学分析。