Investigating cAMP in Taste Transduction

研究 cAMP 在味觉传导中的作用

• 批准号: 7156456
• 负责人: Craig D Roberts
• 金额: $ 3.41万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7156456
• 关键词: adenylate cyclase; calcium flux; cyclic AMP; enzyme activity; enzyme inhibitors; fluorescence resonance energy transfer; fluorescent dye /probe; food flavor; immunocytochemistry; inhibitor /antagonist; intermolecular interaction; isozymes; laboratory mouse; laboratory rat; molecular /cellular imaging; neural information processing; phosphodiesterases; predoctoral investigator; second messengers; single cell analysis; stimulant /agonist; stimulus /response; taste; taste buds

DESCRIPTION (provided by applicant): There is unequivocal evidence from biochemistry and physiology that the diffusible second messenger 3'- 5'cyclic adenosine monophosphate (cAMP) is involved in taste transduction. In contrast, recent reports have emphasized the role of Ca2+ signaling in taste transduction (i.e. Receptor --> G protein --> PLCbeta2 --> delta[Ca2+]i). The significance of the original cAMP data is presently unresolved. The goal of my research is to re-investigate cAMP in taste transduction in the light of current understanding. I propose to image real-time changes in intracellular cAMP using novel, genetically-encoded cAMP reporters. These FRET-based reporters permit one to measure single-cell cAMP levels with excellent spatial and temporal resolution. This work will help define taste signaling at the single cell level and enhance our understanding of how gustatory information is processed and coded within taste buds. Specifically, I postulate that cAMP levels in subsets of individual taste receptor cells are altered in response to gustatory stimuli and that cAMP changes are mutually interactive with Ca2+ fluctuations.

描述（由申请人提供）：生物化学和生理学有明确的证据表明，可扩散的第二信使3'-5'环单磷酸腺苷（cAMP）参与味觉传导。相比之下，最近的报告强调了 Ca2+ 信号在味觉转导中的作用（即受体 --> G 蛋白 --> PLCbeta2 --> delta[Ca2+]i）。原始 cAMP 数据的意义目前尚未确定。我的研究目标是根据目前的理解重新研究 cAMP 在味觉传导中的作用。我建议使用新型基因编码 cAMP 报告基因对细胞内 cAMP 的实时变化进行成像。这些基于 FRET 的报告基因允许人们以优异的空间和时间分辨率测量单细胞 cAMP 水平。这项工作将有助于在单细胞水平上定义味觉信号，并增强我们对味蕾中味觉信息如何处理和编码的理解。具体来说，我假设单个味觉受体细胞亚群中的 cAMP 水平会响应味觉刺激而改变，并且 cAMP 变化与 Ca2+ 波动相互影响。