Multistep evolution of Drosphilia pigment characters

果蝇色素特征的多步进化

• 批准号: 7454224
• 负责人: Mark J Rebeiz
• 金额: $ 4.88万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7454224
• 关键词: Area; Candidate Disease Gene; Color; Complex; Daily; Development; Dissection; Drosophila genus; Drosophila melanogaster; Ectopic Expression; Evolution; Exhibits; Gene Expression; Gene Silencing; Gene Transfer Techniques; Genes; Genetic; Genetic Programming; Human; Life; Melanins; Molecular; Nature; Pathway interactions; Pattern; Pigmentation physiologic function; Pigments; Primates; Race; Relative (related person); Role; Surveys; Testing; Tissues; Wing; base; gene function; insight; novel; trait

DESCRIPTION (provided by applicant): The evolution of form is one of the most awe-inspiring aspects of multicellular life. From the vast array of colors in nature to the changes that brought about the human race from primates, we are confronted with its aftereffects on a daily basis. A particularly important question is how the multiple components of a genetic program are modified to create a new trait. The patterned pigmentation of Drosophila wings presents an exceptional opportunity to dissect the evolution of multigene networks in the origin of complex traits. The aim of this proposal is to identify genes involved in the melanin synthesis pathway which contribute to the evolution of new pigment patterns, and understand how they were co-opted. Once identified, the molecular underpinnings of how each gene has attained a new activity will be explored. A panel of potential genes will be analyzed for expression associated with patterned areas. Using transgenesis of Drosophila melanogaster, the basis of each positive candidate's new activity will be defined. Finally, the sufficiency of identified components will be tested by ectopic expression in a species whose wings are unpatterned.

描述（由申请人提供）：形式的进化是多细胞生命最令人敬畏的方面之一。从自然界多种多样的颜色到灵长类动物给人类带来的变化，我们每天都面临着它的后果。一个特别重要的问题是如何修改遗传程序的多个组成部分以创造新的性状。果蝇翅膀的图案化色素沉着为剖析复杂性状起源中多基因网络的进化提供了绝佳的机会。该提案的目的是识别参与黑色素合成途径的基因，这些基因有助于新色素模式的进化，并了解它们是如何被选择的。一旦确定，将探索每个基因如何获得新活性的分子基础。将分析一组潜在基因的与图案区域相关的表达。利用黑腹果蝇的转基因，将定义每个阳性候选者的新活性的基础。最后，将通过翅膀无图案的物种中的异位表达来测试已识别成分的充分性。