SALIVARY PROTEINS ASSOCIATED W/ ORAL ANTI FUNGICIDAL ACTIVITY

与口服抗真菌活性相关的唾液蛋白

• 批准号: 7369222
• 负责人: Frank G Oppenheim
• 金额: $ 0.05万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369222
• 关键词: SALIVARY; PROTEINS; ASSOCIATED; ORAL; ANTI

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Proteins secreted by the parotid glands are significant components of saliva. These proteins have a variety of known functions including involvement in digestion and prevention of bacterial and fungicidal growth. These studies examine the components of human saliva which have anti-fungicidal activity. Proteins are partially purified by HPLC, affinity and ion exchange chromatography. Fractions demonstrating anti-fungicidal activity are used for protein and mass spectral analysis. Using MALDI-TOF and triple quadrupole MS, several proteins including the histatins and statherin have been identified as having anti-fungicidal activity. It is also apparent that a salivary protein complex, as compared to individual proteins, demonstrates higher anti-fungicidal activity. The protein components of this active complex are being identified and structurally characterized using proteomic techniques, including in-gel protease digestion and tandem MS.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。腮腺分泌的蛋白质是唾液的重要组成部分。这些蛋白质具有多种已知功能，包括参与消化和预防细菌和杀真菌生长。这些研究检查了具有抗牙性活性的人类唾液的成分。蛋白质通过HPLC，亲和力和离子交换色谱法部分纯化。证明抗牙作用活性的分数用于蛋白质和质谱分析。使用MALDI-TOF和三倍四倍体MS，已确定包括组蛋白和Statherin在内的几种蛋白质被确定为具有抗神经毒素活性。同样，显然，与单个蛋白质相比，唾液蛋白复合物表现出较高的抗神经毒素活性。使用蛋白质组学技术（包括凝胶蛋白酶消化和串联MS）鉴定并在结构上鉴定出这种活性复合物的蛋白质成分。