DNA and Phytochemical Fingerprinting of Actaea Species

Actaea 物种的 DNA 和植物化学指纹图谱

• 批准号: 7095402
• 负责人: EDWARD JAMES KENNELLY
• 金额: $ 17.13万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7095402
• 关键词: Asians; DNA; dietary supplements; genetics; restriction fragment length polymorphism

DESCRIPTION (provided by applicant): Black cohosh (Actaea racemosa, syn. Cimicifuga racemosa) is a popular dietary supplement and currently one of the most promising alternative approaches to hormone replacement therapy for menopausal women. The root and rhizomes, often collected by wild-crafting, are typically used for botanical preparations. There are several species of Actaea that co-occur with black cohosh in the US, and some of these are difficult to differentiate. In addition, Asian Actaea species are being sold as black cohosh. Good botanical and manufacturing practices are hampered by the lack of adequate data about the botanical identification and phytochemistry of the different species in this genus. This study involves the examination of variation in Actaea at the species, population, and seasonal levels. We plan to use DNA and phytochemical fingerprinting methods to distinguish A. racemosa from closely related Actaea species by examining variation in eight North American and four Asian species by DNA barcoding, high-performance liquid chromatography-photodiode array (HPLC-PDA), and liquid chromatography-mass spectrometry (LC-MS) methods that we have developed in our laboratories. We will fingerprint the distinguishing features of 20 populations of black cohosh using phytochemical and DNA (amplified fragment length polymorphisms) methods. We will also examine the seasonal variation of black cohosh constituents. The data will be used to improve the identification of black cohosh. For both basic research on plant systematics and the safe use of black cohosh, it is important to develop reliable DNA and phytochemical fingerprinting methods to identify Actaea species. Our long-range goals are to improve the quality of black cohosh dietary supplements and understand the variation in Actaea species.

描述（由申请人提供）： 黑升麻（Actaearacemosa，同义词 Cimicifugaracemosa）是一种流行的膳食补充剂，目前是更年期女性激素替代疗法最有前途的替代方法之一。根和根茎通常通过野生采集，通常用于植物制剂。在美国，有几种与黑升麻共生的 Actaea 物种，其中一些很难区分。此外，亚洲Actaea 物种也以黑升麻的形式出售。由于缺乏有关该属不同物种的植物学鉴定和植物化学的足够数据，良好的植物学和生产实践受到阻碍。这项研究涉及检查 Actaea 在物种、种群和季节水平上的变化。我们计划使用 DNA 和植物化学指纹识别方法，通过 DNA 条形码、高效液相色谱-光电二极管阵列 (HPLC-PDA) 和液体色谱法检查 8 个北美和 4 个亚洲物种的变异，以区分总状总状花序和密切相关的 Actaea 物种。我们在实验室开发的色谱-质谱 (LC-MS) 方法。我们将使用植物化学和 DNA（扩增片段长度多态性）方法对 20 个黑升麻种群的显着特征进行指纹识别。我们还将研究黑升麻成分的季节性变化。该数据将用于改进黑升麻的识别。对于植物系统学的基础研究和黑升麻的安全使用，开发可靠的 DNA 和植物化学指纹方法来鉴定 Actaea 物种非常重要。我们的长期目标是提高黑升麻膳食补充剂的质量并了解 Actaea 物种的变异。