GLIAL MEMBRANES AT THE NODE OF RANVIER PREVENT NEURITE OUTGROWTH

RANVIER结处的胶质膜防止神经突生长

• 批准号: 7420807
• 负责人: DAVID R COLMAN
• 金额: $ 0.29万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-20 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7420807
• 关键词: axon; central nervous system; culture; dendrites; myelin; myelin glycoprotein; neuronal guidance; neurons; node of Ranvier; oligodendroglia; proteomics; spinal cord; thinking; axon; central nervous system; culture; dendrites; myelin; myelin glycoprotein; neuronal guidance; neurons; node of Ranvier; oligodendroglia; proteomics; spinal cord; thinking

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) of mammals restricted neurite outgrowth of cultured neurons. Proteomic analysis of these membranes revealed several inhibitors of neurite outgrowth, including the oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but to oligodendroglia-like cells, whose processes converge to form a ring that completely encircles the nodes. In OMgp-null mice, CNS nodes were abnormally wide and collateral sprouting was observed. Nodal ensheathment in the CNS may stabilize the node and prevent axonal sprouting.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。兰维尔的节点是定期放置的，沿髓神经的非甲基轴突段。在这里，我们表明，抑制培养神经元神经突的神经突中枢神经系统（CNS）分离的淋巴结膜。对这些膜的蛋白质组学分析揭示了神经突生长的几种抑制剂，包括少突胶质细胞髓磷脂糖蛋白（OMGP）。在大鼠脊髓中，OMGP并非本地化以压缩髓磷脂，而是针对寡头样细胞，其过程会聚成一个完全包围节点的环。在OMGP-NULL小鼠中，中枢神经系统节点异常宽，并观察到附带发芽。中枢神经系统中的淋巴结包裹可能会稳定节点并防止轴突发芽。