HVEM OPERATION AND IMPROVEMENTS

HVEM 操作和改进

• 批准号: 7357281
• 负责人: DAVID BARNARD
• 金额: $ 0.56万
• 依托单位: WADSWORTH CENTER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2007-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7357281
• 关键词: HVEM; OPERATION; IMPROVEMENTS

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. ABSTRACT: The Albany HVEM is one of only two in the world that are dedicated to biological studies (the other is in Okazaki, Japan). The HVEM has a maximum accelerating voltage of 1200kV, and a point-to-point resolution of about 5¿. It is equipped with a number of specialized specimen holders, built in-house, that are suitable for single and double-tilt tomography up to 75¿ tilt. A side-entry differentially-pumped environmental chamber is also available. An in-house designed intensified video-rate CCD TV system can be used to observe the image with very low electron irradiation, for focusing and following the image during tomographic data collection. Several areas of improvement are envisioned for increasing the usefulness of the HVEM. Among these are computer control of the tilt stage and implementation of cryo-tomography. The video camera used for televiewing is sensitive enough for manual focus and tracking during cryo-tomography at up to about 10,000X, and the existing cryotransfer stage can likely be repaired. HVEM cryo-tomography of plunge-frozen whole cells will complement work done on the IVEM on the periphery of such cells.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一，该子项目和研究者 (PI) 可能已从其他 NIH 来源获得主要资助，因此可以在其他 CRISP 机构中得到体现。列出的是该中心，不一定是研究者的机构 摘要：奥尔巴尼 HVEM 是世界上仅有的两个致力于生物学研究的机构之一（另一个位于日本冈崎）。最大加速电压1200kV，点对点分辨率约5¿它配备了许多内部内置的专用样品架，适用于高达 75° 的单倾斜和双倾斜断层扫描还可以使用侧入差动泵浦环境室，使用内部设计的增强视频速率 CCD 电视系统以非常低的电子辐射观察图像，以便在断层扫描数据收集期间聚焦和跟踪图像。预计将在几个方面进行改进，以提高 HVEM 的实用性，其中包括倾斜台的计算机控制和冷冻断层扫描的实施，用于电视观看的摄像机对于手动对焦和跟踪足够敏感。冷冻断层扫描高达约 10,000X，并且现有的冷冻转移阶段可能会修复冷冻全细胞的 HVEM 冷冻断层扫描，这将补充在此类细胞外围的 IVEM 上所做的工作。