INVESTIGATING NOVEL DNA FLUORSCENCE LABELING PROBES

研究新型 DNA 荧光标记探针

• 批准号: 7373136
• 负责人: KEVIN BURGESS
• 金额: $ 0.14万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7373136
• 关键词: INVESTIGATING; NOVEL; DNA; FLUORSCENCE; LABELING

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. K. Burgess and co-workers have developed several different types of fluorescence probes for DNA labeling containing more than one fluorescent chromophore, which have distinct visible fluorescence spectra and yet have the same high absorption cross sections at a common wavelength. Two types of such molecules have included trial systems involving oligonucleotides with strongly fluorescent groups conjugated to a nucleobase such as thymidine, and rigid, conjugated, fluoresceinated thymidine triphosphates incorporated into DNA. The aim of the current project is to directly measure energy transfer dynamics by two distinct methods: fluorescence upconversion (150 fs resolution) and a two-photon induced fluorescence technique (~100 fs resolution). Recent comparisons with other dual chromophore probes, involving derivatives of rhodamine and fluorescein, have revealed a correlation of the energy transfer rate with the relative orientation of the electronic transition moments of the two chromophores and the axis of the unsaturated group connecting them. These correlations are currently being examined, through studies of both the energy transfer rate and the fluorescence anisotropy of each type of chromophore pair.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。 K. Burgess 及其同事开发了几种不同类型的用于 DNA 标记的荧光探针，其中包含多个荧光发色团，这些探针具有不同的可见荧光光谱，但在共同波长下具有相同的高吸收截面。两种类型的此类分子包括涉及具有与核碱基（例如胸苷）缀合的强荧光基团的寡核苷酸的试验系统，以及掺入DNA中的刚性、缀合的、荧光素化的胸苷三磷酸。当前项目的目标是通过两种不同的方法直接测量能量转移动力学：荧光上转换（150 fs 分辨率）和双光子诱导荧光技术（~100 fs 分辨率）。 最近与其他双生色团探针（涉及罗丹明和荧光素的衍生物）的比较揭示了能量转移速率与两个生色团的电子跃迁矩的相对方向以及连接它们的不饱和基团的轴的相关性。目前正在通过研究每种发色团对的能量转移率和荧光各向异性来检查这些相关性。