ANIONIC LIPIDS AND MITOCHONDRIAL STRUCTURE

阴离子脂质和线粒体结构

• 批准号: 7358111
• 负责人: WILLIAM DOWHAN
• 金额: $ 0.2万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358111
• 关键词: ANIONIC; LIPIDS; MITOCHONDRIAL; STRUCTURE

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Dowhan's group has isolated mutants of Saccharomyces cerevisiae that lack the mitochondrial specific lipids cardiolipin and phosphatidylglycerol (pgs1) or just lack cardiolipin with increased phosphatidylglycerol (crd1). Pgs1 cells are respiratory deficient, have low mitochondrial membrane potential,and are blocked in the translation of all mitochondrial encoded components of the respiratory chain. They are viable on fermentable carbon sources and we know by light microscopy that their mitochondrial morphology is altered. Crd1 cells grow reasonably well by respiration but we know that the supermolecular complex normally present between Complexes III and IV of the respiratory chain is destabilized in the absense of cardiolipin. This strain also shows grow defects when stressed under respiratory growth conditions. They are interested in detailed morphological information on the structure of the mitochondria of both cells types. In particular tomagraphic reconstruction of mitochondria and their cristae is of interest. If organization of complexes along the cristae is possible, this would also be of interest to correlate with our biochemical data. At the moment they would rely on the NCMIR personel to carry out the studies -- although given protocols by NCMIR, samples would be prepared in Houston.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。 Dowhan 的研究小组分离出了酿酒酵母突变体，这些突变体缺乏线粒体特异性脂质心磷脂和磷脂酰甘油 (pgs1)，或者只是缺乏心磷脂和增加的磷脂酰甘油 (crd1)。 Pgs1细胞呼吸缺陷，线粒体膜电位低，并且呼吸链的所有线粒体编码成分的翻译被阻断。它们在可发酵碳源上是可行的，我们通过光学显微镜知道它们的线粒体形态发生了改变。 Crd1 细胞通过呼吸生长得相当好，但我们知道，通常存在于呼吸链复合物 III 和 IV 之间的超分子复合物在没有心磷脂的情况下会不稳定。当在呼吸生长条件下受到压力时，该菌株也表现出生长缺陷。他们对两种细胞类型线粒体结构的详细形态信息感兴趣。特别是线粒体及其嵴的断层摄影重建令人感兴趣。如果沿着嵴组织复合物是可能的，那么这也将与我们的生化数据相关联。目前，他们将依靠 NCMIR 人员来进行研究——尽管按照 NCMIR 的协议，样本将在休斯顿准备。