TRANSGENIC & TARGETED MUTANT MICE PRESERVATION: BREEDING COLONIES: CRYOPRESERVE

转基因

• 批准号: 7391976
• 负责人: MURIEL T DAVISSON
• 金额: $ 27.08万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-12-01 至 2006-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7391976
• 关键词: TRANSGENIC; TARGETED; MUTANT; MICE; PRESERVATION

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Regarding AIDS This competing renewal seeks continued funding for The Jackson Laboratory''''''''s (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of transgenic and targeted mutation mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific production and use of induced mutant mice generated by these means has rapidly expanded, increasing the need for a resource to maintain, preserve, and distribute these mutant mice at a high health status and with minimal restrictions. Researchers frequently receive requests for mice they have created and want to believed of the burden that distributing mice to other scientists places on their research programs and grants. TJL, founded in 1929 as a non-profit research institution, has maintained and distributed mutant strains of mice for nearly 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is compelling and evidence by its rapid growth. Since it began, the IMR has accepted 495 mutant stocks, is distributing 299 of these, with another 58 currently in the importation process. In 1997, nearly 60,000 mice were distributed. We request support for rederivation, cryopreservation, and strain development, and strain development to improve the scientific value of strains in the IMR; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to: (1) develop methods to assure pathogen-free cyropreservation of mouse sperm, (2) phenotypically characterize new induced mutations and mutations transferred to new congenic genetic backgrounds, and (3) develop more cost-effective genotyping methods. Demand for IMR mice continues to increase, coincident with a decline in funding as several non-renewable start-up grants terminate. The ratio of rejected to accepted strains is increasing (see Introduction). The size of the need is beyond the scope of the NCRR alone to fund. NIAID and NIAMS already contribute to this effort by Intra-Agency Agreements. During the first 4 years of the current grant, over 70% of the mice supplied by the IMR were received by NIH intramural and extramural grantees of 16 NIH Institutes. To address the growing need we request approval of an expansion of this national resource for transgenic and targeted mice to authorize the NCRR to seek additional funding from NIH institutes. To aid in review we have subdivided the overall budget components. The Introduction explains the current funding mechanisms and our proposal to expand the IMR without an excessive burden to the NCRR.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是中心的机构，不一定是研究者的机构。关于艾滋病 这项竞争性的更新寻求继续为杰克逊实验室 (TJL) 的诱导突变资源 (IMR) 提供资金，以继续向科学界提供转基因和定向突变小鼠的重要资源。通过基因转移（转基因小鼠）、同源重组以及时间和组织特异性生产以及通过这些手段产生的诱导突变小鼠的使用选择性地改变小鼠基因组，增加了对维持、保存和分发资源的需求这些突变小鼠健康状况良好，受到的限制最少。研究人员经常收到他们创造的老鼠的请求，并希望相信将老鼠分配给其他科学家会给他们的研究项目和资助带来负担。 TJL 成立于 1929 年，是一家非营利性研究机构，近 50 年来一直维护和分发突变小鼠品系。 1992年，TJL建立了IMR，其目的是进口、冷冻保存和分发具有生物医学重要性的诱导突变小鼠。对 IMR 的需求十分迫切，其快速增长也证明了这一点。自启动以来，IMR 已接受 495 个突变体库存，正在分发其中 299 个，另外 58 个目前正在进口过程中。 1997年，共发放小鼠近6万只。我们请求支持再衍生、冷冻保存和菌株开发，以及菌株开发以提高IMR中菌株的科学价值；小鼠的饲养是为了在尽可能收回成本的基础上分发给其他科学家。为了改进 IMR 资源计划，我们建议进行研究：（1）开发方法以确保小鼠精子的无病原体冷冻保存，（2）表型表征新诱导突变和转移到新同源遗传背景的突变，以及（3）开发更具成本效益的基因分型方法。对 IMR 小鼠的需求持续增加，与此同时，由于一些不可再生的初创企业资助终止，资金减少。拒绝菌株与接受菌株的比例正在增加（参见引言）。需求规模超出了 NCRR 单独资助的范围。 NIAID 和 NIAMS 已经通过机构内协议为这项工作做出了贡献。在当前资助的前 4 年中，超过 70% 的 IMR 提供的小鼠被 16 个 NIH 研究所的 NIH 校内和校外受资助者接收。为了满足日益增长的需求，我们请求批准扩大转基因和靶向小鼠的国家资源，以授权 NCRR 向 NIH 机构寻求额外资金。为了帮助审查，我们对总体预算组成部分进行了细分。引言解释了当前的融资机制以及我们在不给 NCRR 带来过多负担的情况下扩大 IMR 的建议。