Core--Genomics/Proteomics Shared Resources

核心--基因组学/蛋白质组学共享资源

• 批准号: 7525336
• 负责人: Kenneth B Beckman
• 金额: $ 25.32万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7525336
• 关键词: biomedical facility; functional /structural genomics; microarray technology; polymerase chain reaction; proteomics

DESCRIPTION (provided by applicant): Genomics/Proteomics Shared Resources Core Co-Directors: Member: Kenneth Beckman, CHORI Jim Kaput, UC - Davis Dr. Mark Shigenaga, CHORI A, OVERVIEW The Genomics/Proteomics/Analytical Shared Resources Core (henceforth: the "genomics facility core") will centralize and streamline routine genomic, proteomic, and small molecule assays for members of the Minority Disparities Center Grant. Primary services offered will focus on genomics/proteomics and will include SNP genotyping, the analysis of gene expression by microarray and real-time PCR, and 2-D/MALDI-TOF protein characterization. In addition to providing a defined offering of research services, the core staff will supervise access to core equipment for non-routine research by any Center member. The rationale for centralizing such work is to leverage the large investments recently made in existing instrumentation at CHORI, and to minimize duplication of effort in member labs. Specifically, the core budget will primarily be used to support the salaries of two highlyskilled research associates, who will provide services using existing equipment. The core's two codirectors (Dr. Shigenaga and Dr. Beckman) will each supervise one technician, and will provide nonoverlapping functional expertise. Part of the budget will be used to provide on-going maintenance contracts for core instrumentation, to purchase consumables for use in validating and developing protocols for Center members, and to provide software and consumables for sample handling. Details of the objectives, rationale, facilities and equipment, capabilities, staffing, management and usage, and budget of the core are described below.

描述（由申请人提供）： 基因组学/蛋白质组学共享资源核心 联合主任： 成员：Kenneth Beckman，CHORI Jim Kaput，UC - Davis Mark Shigenaga 博士，CHORI A，概述 基因组学/蛋白质组学/分析共享资源核心（此后： “基因组学设施核心”）将集中和简化少数民族差异中心成员的常规基因组、蛋白质组和小分子检测 授予。提供的主要服务将侧重于基因组学/蛋白质组学，包括 SNP 基因分型、通过微阵列和实时 PCR 进行的基因表达分析以及 2-D/MALDI-TOF 蛋白质表征。除了提供明确的研究服务外，核心工作人员还将监督任何中心成员使用核心设备进行非常规研究。集中此类工作的理由是利用 CHORI 最近对现有仪器进行的大量投资，并尽量减少成员实验室的重复工作。具体来说，核心预算将主要用于支持两名高技能研究助理的工资，他们将使用现有设备提供服务。核心的两位联合导演 （Shigenaga 博士和 Beckman 博士）将各自监督一名技术人员，并将提供不重叠的功能专业知识。部分预算将用于提供核心仪器的持续维护合同，购买用于中心成员验证和开发方案的消耗品，以及提供用于样品处理的软件和消耗品。核心的目标、理由、设施和设备、能力、人员配置、管理和使用以及预算的详细信息如下所述。