Immunoprofiling of older persons

老年人的免疫分析

• 批准号: 6884349
• 负责人: Andrzej K Drukier
• 金额: $ 9.98万
• 依托单位: BIOTRACES, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2006-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6884349
• 关键词: beta antiadrenergic agent; biomarker; cardiovascular disorder; clinical research; cytokine; diagnosis design /evaluation; diagnosis quality /standard; enzyme linked immunosorbent assay; female; human middle age (35-64); human old age (65+); human tissue; immune response; immunologic assay /test; inflammation; interferon gamma; interleukin 1; interleukin 10; interleukin 11; interleukin 18; interleukin 4; interleukin 6; interleukin 8; protein quantitation /detection; tumor necrosis factor alpha; women' s health

DESCRIPTION (provided by applicant): Abstract: Autoimmune diseases and older people with weakened immune system are a major public health problem; for example, there are about 2 million individuals with RA, i.e. about 1 % of population of USA. Similarly, there are millions of older people with cardiovascular diseases. Better information is needed about the immunoprofiles of older persons. The specific aims of the proposed Phase I studies are to study the age dependent changes in two cohorts of older women: healthy and with cardiac problems. The last group will be divided into two sub-groups: those treated with beta blockers and those not treated. More specifically, the aims are: Aim 1: Develop the supersensitive panel of assays targeting cytokines in older persons; i.e. further improve immunoassays for pro-inflammatory cytokines (TNF-alpha, IFN-gamma, IL-1 beta and IL-6) and anti-inflammatory cytokines (IL-4, IL-10, IL-11); Aim 2: Develop the super-sensitive assay for IL-8 and IL-18; Aim 3: Study the differences in immunoprofiles between women who are healthy and who have cardiac disorders. Phase II will continue the clinical validation of assays developed in the Phase I effort, e.g. we will develop supersensitive assays targeting five additional cytokines. MPD enhanced P-chips (P-chips/MPD) will be used to measure all relevant biomarkers concurrently in a low cost, supersensitive modality. We will optimize the sensitivity, accuracy and user-friendliness of the P-chip/MPD. Concurrent quantitation of cytokines and other low level biomarkers will permit studies of correlation between the immune system and cardiac output.

描述（由申请人提供）： 摘要：自身免疫性疾病和老年人免疫系统减弱是一个重大的公共卫生问题。例如，大约有 200 万人患有 RA，约占美国人口的 1%。同样，还有数百万老年人患有心血管疾病。需要有关老年人免疫谱的更好信息。 拟议的第一阶段研究的具体目的是研究两组老年女性的年龄依赖性变化：健康女性和有心脏问题的女性。最后一组将分为两个亚组：用β受体阻滞剂治疗的组和未治疗的组。更具体地说，目标是： 目标 1：开发针对老年人细胞因子的超灵敏检测方法；即进一步改进促炎细胞因子（TNF-α、IFN-γ、IL-1β和IL-6）和抗炎细胞因子（IL-4、IL-10、IL-11）的免疫测定； 目标2：开发IL-8和IL-18的超灵敏检测方法； 目标 3：研究健康女性和患有心脏病的女性之间免疫特征的差异。 第二阶段将继续对第一阶段工作中开发的检测方法进行临床验证，例如我们将开发针对另外五种细胞因子的超灵敏测定法。 MPD 增强型 P-chips (P-chips/MPD) 将用于以低成本、超灵敏的方式同时测量所有相关生物标志物。我们将优化 P-chip/MPD 的灵敏度、准确性和用户友好性。细胞因子和其他低水平生物标志物的同时定量将允许研究免疫系统和心输出量之间的相关性。