RHESUS MACAQUE HEMATOPOIETIC STEM CELL EXPANSION

恒河猴造血干细胞扩增

• 批准号: 7349455
• 负责人: PEIMAN HEMATTI
• 金额: $ 2.72万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2007-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7349455
• 关键词: RHESUS; MACAQUE; HEMATOPOIETIC; STEM; CELL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. To initiate pilot studies on the differentiation potential of rhesus macaque embryonic stem cells into hematopoietic stem cells. Hematopoietic stem cells (HSCs) have been used in bone marrow transplantation for treatment of a variety of hematological malignancies for the last two decades. One of the major problems in HSC transplantation is lack of suitable HSC donors. Embryonic stem cells (ESCs) have been proposed as a potentially novel source of HSCs in the future. Nonhuman primates have been proven to be invaluable as a preclinical model for HSC transplantation research. We are planning to develop in vitro and in vivo models of differentiation of rhesus ESCs into HSCs. We are currently at the in vitro phase of our studies. We are receiving peripheral blood and bone marrows samples from rhesus animals through WNRPC and use them as controls and for optimization studies. This research used WNPRC Stem Cell Resources and Animal Services.

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中出现。列出的机构是中心的机构，不一定是研究者的机构。启动恒河猴胚胎干细胞向造血干细胞分化潜力的初步研究。 过去二十年来，造血干细胞（HSC）已被用于骨髓移植，以治疗多种血液恶性肿瘤。 HSC移植的主要问题之一是缺乏合适的HSC供体。胚胎干细胞 (ESC) 已被提议作为未来 HSC 的潜在新来源。非人灵长类动物已被证明作为造血干细胞移植研究的临床前模型具有无价的价值。我们计划开发恒河猴 ESC 分化为 HSC 的体外和体内模型。我们目前正处于研究的体外阶段。我们通过 WNRPC 接收恒河猴的外周血和骨髓样本，并将其用作对照和优化研究。这项研究使用了 WNPRC 干细胞资源和动物服务。