Virus-Mosquito mRNA Stability
病毒-蚊子 mRNA 稳定性
基本信息
- 批准号:7379942
- 负责人:
- 金额:$ 33.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2005
- 资助国家:美国
- 起止时间:2005-06-01 至 2010-02-28
- 项目状态:已结题
- 来源:
- 关键词:3&apos Untranslated RegionsAddressAedesArbovirusesAreaBiological AssayBiologyBunyaviridaeCell ExtractsCellsCulicidaeCytoplasmDataDengueDevelopmentDisease VectorsElementsEnzymesExonucleaseFamilyFlaviviridaeGene ExpressionGenomicsGoalsHandIn VitroIndiumInsectaKineticsKnowledgeLacrosseLife Cycle StagesMammalian CellMediatingMessenger RNAModelingMolecular BiologyNumbersOrganismPathway interactionsPlayPoly APost-Transcriptional RegulationProcessQuality ControlRNA VirusesRegulationRegulatory ElementRoleSeriesSindbis VirusSystemTherapeuticTogaviridaeTranscriptUntranslated RegionsViralVirusWorkbasecomparativeexperiencein vivoinsightmRNA DecaymRNA Stabilitynovelpathogenresearch studytherapeutic targetvectorvector mosquitoviral RNAvirus host interaction
项目摘要
DESCRIPTION (provided by applicant): Mosquitoes are vectors of multiple viral pathogens, a large number of which are RNA viruses. Strategies for viral control may target the vector itself or the interaction between the virus and the vector. This application is based upon the hypothesis that RNA viruses have evolved specific mechanisms for evading the mRNA turnover machineries of the mosquito cell and therefore mRNA decay factors may represent a novel target for therapeutics. Our current knowledge of mRNA decay in insects is minimal thus the primary goals of this proposal must be to elucidate the factors and pathways involved in mRNA turnover in the Aedes mosquito and characterize their regulation. Using an in vitro approach that we have successfully adapted to mosquito cell extracts along with in vivo assays, the goal of Aim I is to characterize the processes of mRNA deadenylation, decapping and decay in mosquitoes. In Aim II we will use this knowledge to gain insights into mechanisms of regulated mRNA decay mediated by togavirus 3' untranslated regions that we have observed. The final aim of the application will address the underlying mechanisms responsible for how non- polyadenylated viral RNAs avoid degradation upon entry into the mosquito cell. In summary, this information will provide fundamental insights into insect molecular biology and virus-host interactions that will provide the groundwork for novel avenues of arbovirus control.
描述(由申请人提供):蚊子是多种病毒病原体的向量,其中大量是RNA病毒。病毒控制的策略可以针对向量本身或病毒与载体之间的相互作用。该应用是基于以下假设:RNA病毒已经发展出了逃避蚊子细胞的mRNA离职机制的特定机制,因此mRNA衰减因子可能代表了治疗剂的新靶标。我们目前对昆虫中mRNA衰变的了解很少,因此该提案的主要目标必须是阐明埃德斯蚊子中mRNA转换涉及的因素和途径,并表征其调节。使用一种体外方法,我们成功地适应了蚊子提取物以及体内测定,目标I的目的是表征mRNA Deadenylation,Decapping和decay的过程。在AIM II中,我们将使用这些知识来了解我们观察到的togavirus 3'未翻译区域介导的受调节mRNA衰变的机制。该应用的最终目的将解决负责非聚腺苷酸化病毒RNA如何避免进入蚊子细胞降解的潜在机制。总而言之,这些信息将提供对昆虫分子生物学和病毒宿主相互作用的基本见解,这将为新型Arbovirus Control提供基础。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Jeffrey Wilusz其他文献
Jeffrey Wilusz的其他文献
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{{ truncateString('Jeffrey Wilusz', 18)}}的其他基金
Pathological Implications of Repression of Cellular RNA Decay by Zika Virus
寨卡病毒抑制细胞 RNA 衰变的病理学意义
- 批准号:
9298165 - 财政年份:2017
- 资助金额:
$ 33.72万 - 项目类别:
Flavivirus non-coding RNAs and the Host mRNA Decay Machinery
黄病毒非编码 RNA 和宿主 mRNA 衰变机制
- 批准号:
9356456 - 财政年份:2016
- 资助金额:
$ 33.72万 - 项目类别:
Flavivirus non-coding RNAs and the Host mRNA Decay Machinery
黄病毒非编码 RNA 和宿主 mRNA 衰变机制
- 批准号:
9762831 - 财政年份:2016
- 资助金额:
$ 33.72万 - 项目类别:
Flavivirus non-coding RNAs and the Host mRNA Decay Machinery
黄病毒非编码 RNA 和宿主 mRNA 衰变机制
- 批准号:
9238132 - 财政年份:2016
- 资助金额:
$ 33.72万 - 项目类别:
A novel antiviral approach using the cellular RNA decay machinery
一种利用细胞 RNA 衰变机制的新型抗病毒方法
- 批准号:
8261431 - 财政年份:2011
- 资助金额:
$ 33.72万 - 项目类别:
A novel antiviral approach using the cellular RNA decay machinery
一种利用细胞 RNA 衰变机制的新型抗病毒方法
- 批准号:
7675653 - 财政年份:2009
- 资助金额:
$ 33.72万 - 项目类别:
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