Proteomics

蛋白质组学

• 批准号: 7516324
• 负责人: William Steven Dalton
• 金额: $ 16.4万
• 依托单位: H. LEE MOFFITT CANCER CTR & RES INST
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-02-01 至 2012-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7516324
• 关键词: Basic Science; Biological Markers; Biometry; Cancer Center; Clinical; Collaborations; Community Health Education; Complex; Core Facility; Coupled; Elements; Environment; Gel; Goals; Individual; Informatics; Internet; Investigation; Label; Left; Liquid Chromatography; Mass Spectrum Analysis; Modification; Pathway interactions; Peptides; Phosphoproteins; Population; Post Translational Modification Analysis; Post-Translational Protein Processing; Protein Chemistry; Proteins; Proteome; Proteomics; Publications; Research; Research Project Grants; Resources; Sampling; Series; Services; Silver Staining; Site; Staining method; Stains; Training; Translational Research; Two-Dimensional Gel Electrophoresis; Tyrosine; assay development; base; data mining; design; foot; member; novel; protein aminoacid sequence; research study; tandem mass spectrometry

The Proteomics Core Facility provides an interactive resource for protein chemistry, separations, and mass spectrometry. The Proteomics Core Facility serves the research needs of basic science with specifically targeted functional investigations, including the identification of interacting proteins isolated by coimmunoprecipitation, localization of post-translational modifications, and development of assays for enzymatic activity. For clinical and translational projects, the Proteomics Core Facility offers profiling services, using either 2D gel electrophoresis or liquid chromatography coupled to mass spectrometry, and subsequent identification of relevant proteins indicated by data mining. In addition, community education is a key element of developing this new facility at Moffitt Cancer Center; training is provided through a series of seminars and resources are made available on an internal Web site.

蛋白质组学核心设施为蛋白质化学，分离和质量提供了交互式资源 光谱法。蛋白质组学核心设施特别适合基础科学的研究需求 有针对性的功能研究，包括鉴定由由 共免疫沉淀，翻译后修饰的定位和开发用于开发 酶活性。对于临床和翻译项目，蛋白质组学核心设施提供了分析 服务，使用2D凝胶电泳或液态色谱法耦合到质谱，并使用 随后鉴定数据挖掘指示的相关蛋白质。此外，社区教育是 在莫菲特癌症中心开发新设施的关键要素；通过一系列培训 研讨会和资源可在内部网站上提供。