CORE--ELECTRON MICROSCOPY

核心--电子显微镜

• 批准号: 7406614
• 负责人: George B Witman
• 金额: $ 5.34万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7406614
• 关键词: Arts; Charge; Clinical Sciences; Core Facility; Cryoelectron Microscopy; Cryoultramicrotomy; Electron Microscope; Electron Microscopy; Electrons; Equipment; Freeze Etching; Freeze Fracturing; Freeze Substitution; Freezing; Funding; Goals; Image; Immunoelectron Microscopy; Metals; Methodology; Methods; Microtomy; Negative Staining; Paper; Preparation; Printing; Publishing; Rate; Reagent; Reporting; Research; Research Personnel; Scanning; Scanning Electron Microscopy; Services; Shadowing (Histology); Specimen; Spectrum Analysis; System; Techniques; Time; Transmission Electron Microscopy; Work; base; cost; day; enterotoxigenic Escherichia coli; experience; instrumentation; member; transmission process

The goal of the DERC Electron Microscopy (EM) Core Facility is to provide complete, first-rate EM service to DERC researchers at UMMS. The Facility is centrally located in a newly renovated suite in the basement of the Basic and Clinical Sciences Building. It is directed by Drs. George Witman and Roger Craig, and managed on a day-to-day basis by Dr. Gregory Hendricks, a full-time professional electron microscopist with extensive experience in virtually all EM methods. Equipment includes Philips CM120, CM12 and CM10 transmission electron microscopes and an ETEC Autoscan scanning electron microscope, a Balzers BAF400T freeze-etching system; three Reichert Ultracut E cryo-ultramicrotomes, and other equipment for specimen preparation. Techniques include immuno-EM, cryo-EM, and preparation of specimens by conventional thin sectioning, negative staining, metal shadowing, cryoultramicrotomy, rapid freezing, freeze substitution, freeze-fracture/freeze-etch, and critical point drying. Dr. Hendricks, aided by an assistant, can provide all services from specimen preparation to printing of electron micrographs. Alternatively, investigators may use Facility reagents and equipment to carry out their own specimen preparation and examination, with assistance from Facility staff as needed. Consequently, the Facility works just as well for users with no EM experience as for those with considerable EM experience. By centralizing all aspects of EM in this facility, the DERC provides its members with the best possible EM services in the most cost- and time-effective manner. The Facility, which is open to all UMMS researchers, is supported by a combination of institutional funds and user chargebacks; DERC investigators are charged at one-half the rate for non-affiliated UMMS researchers. Many DERC members use EM as an integral part of their research, and DERC members have published numerous papers that report results obtained in the EM Core Facility.

DERC电子显微镜（EM）核心设施的目的是为UMMS的DERC研究人员提供完整的一流EM服务。该设施位于基础和临床科学大楼地下室的新装修套件中。它由Drs执导。乔治·维特曼（George Witman）和罗杰·克雷格（Roger Craig），并由Gregory Hendricks博士每天进行管理，Gregory Hendricks博士是一位全职专业电子显微镜，在几乎所有EM方法方面都有丰富的经验。设备包括Philips CM120，CM12和CM10传输电子显微镜以及ETEC Autoscan扫描电子显微镜，Balzers BAF400T Freeze-etching系统；三个Reichert Ultracut E冷冻粉状细菌和其他用于样品制备的设备。技术包括免疫EM，冷冻EM以及常规薄的标本制备 切片，负面染色，金属阴影，冰冻曲霉切开术，快速冻结，冻结替代，冻结裂缝/冻结 - 蚀刻和临界点干燥。在助手的帮助下，亨德里克斯博士可以提供从标本准备到电子显微照片的所有服务。另外，调查人员可以根据需要在设施人员的协助下使用设施和设备进行自己的标本准备和检查。因此，对于没有EM经验的用户和具有丰富经验的用户，该设施也同样有效。通过将EM的各个方面集中在此设施中，DERC 以最具成本及时的方式为其成员提供最佳的EM服务。该设施向所有UMMS研究人员开放，并由机构资金和用户退款的组合支持； DERC研究人员的收费是非附属UMMS研究人员的一半。许多DERC成员将EM用作研究的组成部分，DERC成员发表了许多论文，报告了在EM核心设施中获得的结果。