FUNDS TO ACQUIRE A JEOL JSM-7401F FESEM: INFECTIOUS DISEASE

获得 JEOL JSM-7401F FESEM 的资金：传染病

• 批准号: 7335217
• 负责人: Michael A. Apicella
• 金额: $ 26.68万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335217
• 关键词: human; infection; microscopy; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds for a JEOL JSM-7401F Field Emission Scanning Electron Microscope with embedded digital imaging, beam decelerating technology and high resolution backscattered electron imaging. The University of Iowa has one SEM (a Hitachi S-4000 acquired in 1989) to serve the entire campus. The current system cannot provide images of immunogold labeled samples at low accelerating voltages and a second system is critically needed because having only one system is severely limiting access by investigators. From February 1, 2004 to January 31, 2005, 87 investigators from 54 faculty labs in 22 departments and 6 colleges used the S-4000 SEM. 30 of the 54 faculty labs have NIH funded research grants totaling over $38,000,000.00 annually. To better support this research, it is critical to have ready access to state-of-the-art instrumentation. Heavy usage requires Investigators to reserve the Hitachi S-4000 SEM 2-3 weeks in advance. The system is made available 24 hrs a day to accommodate investigators. In addition, an average of 20 students a semester enroll in formal Microscopy Research classes. SEM short courses and workshops are also offered on a regular basis. Downtime for maintenance on the S-4000 has been nearly 25% and is due to the combination of very heavy use, difficulty in obtaining replacement parts and inexperienced service support. The new FESEM would be conveniently located in room 80 of the Eckstein Medical Research Building under the supervision of The University of Iowa Central Microscopy Research Facility (CMRF). Administration, maintenance as well as high-level technical assistance and training will be provided by experienced staff. Institutional commitment is strong, providing $39,000.00 in salary support for a Research Assistant and $14,000.00 towards the annual service contract on a continuing basis as well as $12,550.00 to upgrade a cryostage to be used on the JEOL JSM-7401 F. The five NIH funded faculty providing research descriptions are the heaviest users of the S-4000 SEM. Apicella will study human pathogenic bacteria. Welsh will investigate human airway epithelia cell biology in relation to Bardet- Biedl syndrome and other diseases. Grose is involved in determining the mechanism of human herpes virus infections. Wilson examines Leismania compromised human macrophages. Starner will study the relationship of Haemophilus biofilms to infection and inflammation in Cystic Fibrosis patients.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：该提案要求使用嵌入式数字成像，梁减速技术和高分辨率反向散射电子成像的JEOL JSM-7401F场发射电子显微镜进行资金。爱荷华大学有一个SEM（1989年获得的Hitachi S-4000）为整个校园服务。当前的系统无法在低加速度的电压下提供免疫金标记的样品的图像，并且非常需要第二个系统，因为只有一个系统严重限制了研究人员的访问。从2004年2月1日到2005年1月31日，来自22个系的54名教职员工和6所大学的87名调查员使用S-4000 SEM。在54个教师实验室中，有30名NIH资助了每年超过38,000,000.00美元的研究补助金。为了更好地支持这项研究，至关重要的是，准备好使用最新的仪器。大量使用要求调查人员提前2-3周保留日立S-4000 SEM。该系统每天24小时可容纳调查人员。此外，每学期平均有20名学生参加正式的显微镜研究课程。还定期提供SEM简短课程和讲习班。在S-4000上进行维护的停机时间接近25％，这是由于非常大量使用的结合，难以获得替换零件以及缺乏经验的服务支持。在爱荷华大学中央显微镜研究机构（CMRF）的监督下，新的FESEM将方便地位于埃克斯坦医学研究大楼80室。经验丰富的员工将提供管理，维护以及高级技术援助和培训。机构承诺很强，为研究助理提供了39,000.00美元的薪水支持，并持续为年度服务合同提供$ 14,000.00，以及$ 12,550.00，以升级jeol JSM-7401 F上使用的低温量。 Apicella将研究人类的致病细菌。威尔士将研究与Bardet-Biedl综合征和其他疾病有关的人类气道上皮细胞生物学。 Grose参与确定人疱疹病毒感染的机制。威尔逊研究利斯尼亚损害了人类巨噬细胞。 Starter将研究嗜血菌生物膜与囊性纤维化患者感染和炎症的关系。