CONFOCAL EQUIPMENT FOR BIOMEDICAL & NANOMEDICINE: CARDIOVASCULAR

生物医学共聚焦设备

• 批准号: 7335261
• 负责人: Tzyh-Chang Hwang
• 金额: $ 4.22万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-20 至 2007-04-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335261
• 关键词: GABA receptor; biomedical equipment; blood pressure; endocytosis; exocytosis; glutamates; housing; ions; lasers; microscopy; nanomedicine; nanotechnology; physiology; receptor; tissues; university; voltage /patch clamp

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal requests funds to purchase a laser scanning confocal microscope system with FRET and TIRF microscopic capabilities. The instrument will be housed within the imaging facility of Dalton Cardiovascular Research Center (DCRC) at the University of Missouri. While several modern fluorescent microscopes are available for general use, we do not have a confocal microscope at this site. There is a high demand placed on the fee-for-service access to the single confocal microscope available at this university creating shortage of large time blocks necessary for conducting live cell/tissue imaging experiments. In view of the time- sensitive nature of our experiments and difficulty in transporting live cells/ tissue to this location, the addition of a confocal microscope to the imaging core at DCRC would greatly enhance a diverse array of ongoing NIH-funded research programs. As detailed in this proposal, an advisory committee was formed to identify a core group of users, determine the specific needs of DCRC and to choose the appropriate instrumentation to accomplish the goals of our research groups. Following extensive consultation and analysis of various competing confocal systems, the Olympus Fluoview 1000 has been identified as the ideal system. This system can be modified to integrate FRET and TIRF capabilities with patch-clamp techniques to serve the needs of investigators applying innovative fluorescent microscopy and nanotechnology approaches to central questions in membrane physiology such as exocytosis/ endocytosis, ion channel gating and receptor- mediated signal transduction. In addition, this confocal system will also support a second group of investigators studying subcellular localization of glutamate and GABA receptors and neuronal pattern generation in the central nuclei involved in blood pressure regulation. The addition of the proposed confocal microscope will not only maximize the impact of our existing research programs but also will help in recruiting new, talented faculty to join the already successful group at our Institution.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：该提案要求资金购买具有FRET和TIRF显微镜功能的激光扫描共聚焦显微镜系统。该乐器将安置在密苏里大学道尔顿心血管研究中心（DCRC）的成像设施中。尽管有几种现代荧光显微镜可用于一般使用，但我们在此站点没有共聚焦显微镜。对在该大学提供的单个共聚焦显微镜的费用访问量的需求很高，从而造成了进行活细胞/组织成像实验所需的大型时间块的短缺。鉴于我们的实验的时间敏感性以及将活细胞/组织运输到该位置的困难，在DCRC处将共聚焦显微镜添加到成像核心上，将大大增强一系列正在进行的NIH资助的研究计划。正如本提案中详细介绍的那样，成立了一个咨询委员会来确定核心用户的核心群体，确定DCRC的特定需求，并选择适当的工具以实现我们研究小组的目标。在对各种竞争共聚焦系统进行广泛的咨询和分析之后，Olympus Fluoview 1000被确定为理想系统。可以对该系统进行修改，以将FRET和TIRF功能与贴片钳技术整合在一起，以满足研究人员的需求，该研究人员将创新的荧光显微镜和纳米技术方法应用于膜生理学中的中心问题，例如胞吞和内吞作用，ION吞噬作用，离子通道和受体介导的信号介导的信号转移。此外，该共聚焦系统还将支持第二组研究人员，研究谷氨酸和GABA受体的亚细胞定位以及与血压调节有关的中央核中的神经元模式产生。拟议的共聚焦显微镜的增加不仅将最大化我们现有的研究计划的影响，而且还将有助于招募新的，才华横溢的教师加入我们机构已经成功的小组。