LSM CONFOCAL SYSTEM: HEARING

LSM 共焦系统：听力

• 批准号: 7335235
• 负责人: JOHN Irwin CLARK
• 金额: $ 10万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-15 至 2007-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335235
• 关键词: fluorescent dye /probe; ionophores; microscopy; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): We are requesting funds to purchase a Zeiss LSM 510 META NLO on an upright epifluorescence Axioplan 2 microscope. Over the last several years, the invention of multiphoton (two-photon laser scanning) microscopy has spearheaded cellular research into new realms, providing real-time views of cell morphology, function and development within the living animal Multiphoton microscopy uses high-energy infrared pulses to excite fluorophores, therefore enabling imaging of structures deep (hundreds of micrometers) within the tissue. Another advantage of multiphoton imaging is that living specimens can be imaged repeatedly over long periods of time (hours to weeks). This is because excitation is effectively confined to the focal point of the beam, such that fluorophores above and below are not excited. Photobleaching and phototoxicity, common limitations of confocal microscopy, are thus much reduced when using multiphoton microscopy. This microscope will be used to support the ongoing, funded research programs of three primary investigators in two different departments at the University of Washington. Although these studies will focus on basic mechanisms of neural development and plasticity, the findings will have implications for research in neuroscience and neurological diseases, ophthalmology and vision research and deafness and auditory dysfunction. The requested instrument would be housed in the Biological Structure Imaging facility, and would be administered and maintained by funds from the department. The proposed administrative structure and management of the instrument are optimized for the best utilization of the microscope time, thus assuring the maximum benefit to the primary investigators, as well as a broad range of secondary users at the University.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：我们要求资金在直立的落射荧光式Axioplan 2显微镜上购买Zeiss LSM 510 meta nlo。在过去的几年中，多光子（两光子激光扫描）显微镜的发明已将细胞研究带到了新领域，从而提供了对活的动物多人显微镜内的细胞形态，功能和发育的实时观点，它使用了高能量的红外脉冲脉冲来使荧光脉动启动荧光液，从而使结构的成年构成了一百多个小组（数量）。多光子成像的另一个优点是，可以在长时间（小时至几周）中反复成像活样本。这是因为激发有效地局限于光束的焦点，因此上方和下方的荧光团并不激发。因此，当使用多光子显微镜检查时，光漂白和光毒性是共聚焦显微镜的常见局限性。该显微镜将用于支持华盛顿大学两个不同系的三个主要研究人员的持续资助的研究计划。尽管这些研究将侧重于神经发育和可塑性的基本机制，但这些发现将对神经科学和神经系统疾病，眼科和视力研究以及耳聋以及听觉功能障碍的研究产生影响。所请求的工具将安置在生物结构成像设施中，并将由部门的资金管理和维护。拟议的行政结构和仪器的管理非常优化，以最佳利用显微镜时间，从而确保了对主要研究人员的最大收益，以及大学的大量二级用户。