Vision Research Infrastructure Development Grant (R24)

愿景研究基础设施发展补助金 (R24)

• 批准号: 7188992
• 负责人: Frank Joseph Giblin
• 金额: $ 21.68万
• 依托单位: OAKLAND UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7188992
• 关键词: Area; Arts; Award; Biochemistry; Biological Sciences; Cancer Center Support Grant; Cataract; Cell Death; Cells; Communities; Confocal Microscopy; DNA; DNA Sequence; Development; Disease; Electron Microscopy; Environment; Equipment; Extracellular Matrix; Eye; Gene Expression; Glaucoma; Grant; Health; Histology; Hospital Departments; Hospitals; Image; Image Analysis; Light; Metabolism; Michigan; Microarray Analysis; Molecular; Molecular Biology; Monoclonal Antibody R24; Ophthalmologist; Ophthalmology; Participant; Photoreceptors; Research; Research Infrastructure; Research Institute; Research Personnel; Retinal; Retinal Degeneration; Role; Scanning Transmission Electron Microscopy Procedures; Sensory; Services; Structure; Techniques; Universities; Vision; Vision research; base; eye center; ganglion cell; lens; programs; tissue culture

DESCRIPTION (provided by applicant): The objective of this R24 application is to enhance the research capabilities of Oakland University's vision researchers, and promote collaborative research among the vision research community within the University and the affiliated William Beaumont Hospital, Department of Ophthalmology. Ten investigators, seven from the Eye Research Institute at O.U. and three from the O.U. Department of Biological Sciences, are designated as participants in the program. Six of the investigators hold independent NEI research awards, and one has a first-award application currently under review. Programs include lens and cataract, retinal biochemistry and metabolism in health and disease, the role of extracellular matrix in ganglion cell death in glaucoma, photoreceptor-related mechanisms of retinal degeneration and coordination of gene expression in retinal development. Support is requested for two Modules, Cell and Molecular Biology, and Ocular Structure and Imaging, in order to reorganize and expand existing Tissue Culture and Electron Microscopy/Histology Modules from a prior NEI Core Grant held by O.U. for the past 16 years. The Cell and Molecular Biology Module will provide assistance to O.U. vision researchers in the areas of tissue culture, basic molecular techniques, DNA sequencing and microarray technology, the latter in conjunction with the Sensory DNA Array Facility at the Kellogg Eye Center, University of Michigan. The Ocular Structure and Imaging Module will enhance the vision research environment of R24 participants by providing state-of-the-art capabilities for ultrastructure and imaging analysis using epifluorescence, routine light and confocal microscopy, as well as scanning and transmission electron microscopy. The availability of the requested facilities on a shared-use basis will greatly increase the vision research capabilities of O.U. investigators as well as Ophthalmologists, Residents and Fellows at Beaumont Hospital, who would otherwise not be able to afford the sophisticated equipment and services associated with the advanced techniques that are described.

描述（由申请人提供）：该R24申请的目的是增强奥克兰大学远见研究人员的研究能力，并促进该大学内视觉研究社区和眼科系关联的William Beaumont医院之间的合作研究。十位调查人员，来自O.U.眼睛研究所的七名和三个来自O.U.生物科学系被指定为该计划的参与者。六名调查人员持有独立的NEI研究奖，其中一项目前正在审查中备受审查。程序包括晶状体和白内障，视网膜生物化学以及在健康和疾病中的代谢，细胞外基质在神经节细胞死亡中的作用在青光眼中，视网膜变性的光感受器相关机制以及基因表达在视网膜发育中的均衡。为了重新组织和扩展O.U.先前的NEI核心拨款的两个模块，即细胞和分子生物学以及眼部结构和成像，请求支持两个模块。在过去的16年中。细胞和分子生物学模块将为O.U提供帮助。在组织培养，基本分子技术，DNA测序和微阵列技术领域的视觉研究人员，后者与密歇根大学Kellogg眼中心的感觉DNA阵列设施结合使用。眼睛结构和成像模块将通过提供最先进的能力来增强R24参与者的视觉研究环境，用于使用Epifluorecens，常规光和共聚焦显微镜以及扫描和透射电子显微镜，用于超微结构和成像分析。在共享的基础上，请求的设施的可用性将大大提高O.U.的愿景研究能力。 Beaumont医院的调查人员以及眼科医生，居民和研究员，否则他们将无法负担与所描述的先进技术相关的复杂设备和服务。

项目成果

Posterior vitreous detachment with microplasmin alters the retinal penetration of intravitreal bevacizumab (Avastin) in rabbit eyes.

• DOI: 10.1097/iae.0b013e3181e586b2
• 发表时间: 2011-02
• 期刊: Retina (Philadelphia, Pa.)
• 作者: Goldenberg DT;Giblin FJ;Cheng M;Chintala SK;Trese MT;Drenser KA;Ruby AJ
• 通讯作者: Ruby AJ

A decrease in phosphorylation of cAMP-response element-binding protein (CREBP) promotes retinal degeneration.

• DOI: 10.1016/j.exer.2011.03.017
• 发表时间: 2011-06
• 期刊: EXPERIMENTAL EYE RESEARCH
• 影响因子: 3.4
• 作者: Mali, Raghuveer S.;Zhang, Xiao M.;Chintala, Shravan K.
• 通讯作者: Chintala, Shravan K.

Expression and purification of his-tagged recombinant mouse zeta-crystallin.

• DOI: 10.1016/j.pep.2009.08.001
• 发表时间: 2010-02
• 期刊: PROTEIN EXPRESSION AND PURIFICATION
• 影响因子: 1.6
• 作者: Simpanya, Mukoma F.;Leverenz, Victor R.;Giblin, Frank J.
• 通讯作者: Giblin, Frank J.

Retinaldehyde, a potent inhibitor of gap junctional intercellular communication.

视黄醛，间隙连接细胞间通讯的有效抑制剂。

• DOI: 10.1080/15419060490471784
• 发表时间: 2004
• 期刊: Cell communication & adhesion.
• 作者: Pulukuri,Sadhona;Sitaramayya,Ari
• 通讯作者: Sitaramayya,Ari

Selective degeneration of central photoreceptors after hyperbaric oxygen in normal and metallothionein-knockout mice.

正常和金属硫蛋白敲除小鼠高压氧后中央光感受器的选择性变性。

• DOI: 10.1167/iovs.07-1039
• 发表时间: 2008-07
• 期刊: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
• 影响因子: 4.4
• 作者: Nachman-Clewner, Michele;Giblin, Frank J.;Dorey, C. Kathleen;Blanks, Robert H. I.;Dang, Loan;Dougherty, Christopher J.;Blanks, Janet C.
• 通讯作者: Blanks, Janet C.