Lesion Analysis Core

病变分析核心

基本信息

批准号：
7160717
负责人：
JAN Leslie BRESLOW
金额：
$ 17.75万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-04-01 至 2011-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7160717
关键词：
atherosclerosis atherosclerotic plaque biomedical facility blood lipoprotein cellular pathology gene expression genetic susceptibility genetically modified animals histopathology laboratory mouse laser capture microdissection pathologic process

项目摘要

The major theme of the PPG is the use of induced mutant mouse models to identify new genes and pathways involved in atherosclerosis. As a consequence, all of the projects in the PPG have as major endpoints the assessment of atherosclerotic lesions. Core 'A' will assist investigators in making 3 types of measurements including: 1) quantitation of cross sectional lesion area in the aortic root and brachiocephalic artery (BCA), 2) quantitation of aortic surface lesion area by the en face method, and 3) qualitative and quantitative assessment of plaque progression phenotypes. The first two of these and some measurements pertaining to the third will be done in Dr. Breslow's laboratory at Rockefeller. Measurements of plaque progression phenotypes will also be done in Dr. Tabas' laboratory at Columbia. In addition, all PPG projects have proposed using Laser Capture Microdissection (LCM) to assess gene expression in various cell types in atherosclerotic lesions. Core 'A' will act as intermediary with Dr. Edward Fisher's Laboratory at New York University Medical Center to train PPG investigators/technicians in the use of the LCM technique. A trained person will then be allowed to use the machinery on a sign up basis. Dr. Fisher will consult/collaborate on each of these projects.

PPG 的主题是利用诱导突变小鼠模型来识别新基因和参与动脉粥样硬化的途径。因此，PPG 中的所有项目都具有主要的终点是动脉粥样硬化病变的评估。核心“A”将协助调查人员制作 3 种类型的测量包括：1) 主动脉根部和头臂横截面病变面积的定量动脉 (BCA)，2) 通过 enface 方法定量主动脉表面病变区域，以及 3) 定性和斑块进展表型的定量评估。其中前两个和一些测量值与第三个相关的研究将在布雷斯洛博士位于洛克菲勒的实验室中完成。菌斑测量进展表型也将在哥伦比亚大学塔巴斯博士的实验室进行。此外，所有PPG项目提出使用激光捕获显微切割 (LCM) 来评估各种细胞类型中的基因表达在动脉粥样硬化病变中。核心“A”将充当纽约爱德华·费舍尔博士实验室的中介大学医学中心培训 PPG 研究人员/技术人员使用 LCM 技术。受过训练的然后，人们将被允许在注册的基础上使用该机器。费舍尔博士将在以下方面进行咨询/合作这些项目中的每一个。