Core A- Informatic Gene Array

核心A-信息基因阵列

• 批准号: 7255608
• 负责人: Daniel J. Garry
• 金额: $ 30.94万
• 依托单位: TEXAS A&M UNIVERSITY HEALTH SCIENCE CTR
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7255608
• 关键词: biomedical facility; cardiogenesis; developmental genetics; embryonic stem cell; flow cytometry; mammalian embryology; microarray technology; molecular biology information system; tissue /cell culture

The objective of this core facility is to provide the technical and scientific support for the research projects through the isolation of rare or specific cell populations, the amplification of RNA isolated from the respective cell populations and the comprehensive analysis of the genetic program of these cell populations using microarray analysis. Specifically, we have established the following expertise, which will be provided for the support of each of the projects. 1) Analysis of cell populations using FACS analysis; 2) RNA micro-isolation; 3) Linear RNA amplification; 4) Microarray analysis; and 5) Bioinformatics of microarray data for the initial analysis and integration of results with previous studies. This is an extension of an ongoing collaboation between Dr. Garry and the project leaders.

该核心设施的目的是通过隔离稀有或特定的细胞群体，从相应的细胞群中分离的RNA的扩增以及使用微阵列分析对这些细胞群体的遗传程序的全面分析来为研究项目提供技术和科学支持。具体来说，我们已经建立了以下专业知识，将提供每个项目的支持。 1）使用FACS分析分析细胞群体； 2）RNA微异化； 3）线性RNA 放大; 4）微阵列分析； 5）微阵列数据的生物信息学用于初步分析和整合结果与先前的研究。这是Garry博士和项目负责人之间正在进行的合作的延伸。