DEVELOPMENT AMYLOID B-PROTEIN OLIGOMERIZATION INHIBITORS

淀粉样 B 蛋白寡聚化抑制剂

• 批准号: 7112795
• 负责人: GAL BITAN
• 金额: $ 24.74万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7112795
• 关键词: Alzheimer' s disease; amyloid proteins; amyloidosis; cell line; chemical structure function; circular dichroism; combinatorial chemistry; drug design /synthesis /production; inhibitor /antagonist; mass spectrometry; molecular pathology; neurofibrillary tangles; neurons; neuropathology; neuropharmacology; neurotoxins; oligopeptides; organic brain syndrome; protein folding; proteolysis

We hypothesize that soluble amyloid beta-protein (Abeta) oligomers are key effectors of neurotoxicity and may be a primary cause of Alzheimer's disease (AD). Consequently, inhibition of Abeta Oligomerization is an attractive strategy for preventing and treating AD. We propose to use a systematic, rational design approach for preparation and structure-activity studies of Abeta Oligomerization inhibitors. We will focus our efforts on inhibitors of early Abeta(1-42) oligomers termed "paranuclei." We choose early Abeta(1-42) oligomers as our primary target because Abeta(1-42) is particularly linked to AD and because inhibition of early assembly of Abeta(1-42) will alleviate the neurotoxic effects, both of the oligomers themselves and of the larger neurotoxic assemblies, protofibrils and fibrils, for which paranuclei are precursors. Our design in based on recent experimental and modeling data that delineate structural features of paranucleus assembly, including primary-quaternary structure relationships and conformation of the C-terminus of Abeta(1-42). This region is responsible directly for the enhanced toxicity and distinct Oligomerization pattern of Abeta(1-42) relative to the more abundant alloform, Abeta(1-40). The inhibitor design process is tightly integrated with the structural and biological projects within the overall Program. The design process not only will benefit from the structural data generated by the Program members, but also will feed back into structural studies and provide further understanding of how particular regions and residues in Abeta interact with each other to form oligomers.

我们假设可溶性淀粉样蛋白β-蛋白质（ABETA）低聚物是神经毒性的关键效应因子和 可能是阿尔茨海默氏病（AD）的主要原因。因此，抑制Abeta寡聚化 是预防和治疗广告的有吸引力的策略。我们建议使用系统的，理性的 用于ABETA低聚抑制剂的制备和结构活性研究的设计方法。我们 我们的努力将集中在Abeta早期（1-42）低聚物的抑制剂上，称为“ paranuclei”。我们尽早选择 Abeta（1-42）低聚物作为我们的主要目标，因为Abeta（1-42）特别与AD相关，因为 抑制Abeta早期组装（1-42）将减轻神经毒性作用，这两种寡聚物 其本身以及较大的神经毒性组件，原纤维和原纤维，副核心为 前体。我们的设计基于最新的实验和建模数据来描述结构 副核心组装的特征，包括初级杂物结构关系和 Abeta的C末端的构象（1-42）。该地区直接负责增强 相对于更丰富的同种异体，ABETA（1-42）的毒性和独特的寡聚模式， Abeta（1-40）。抑制剂设计过程与结构和生物学项目紧密整合 在整个程序中。设计过程不仅将从结构数据中受益 计划成员生成的 进一步了解Abeta中的特定区域和残留物如何相互作用以形成 低聚物。