Prox1 in embryonic and adult lymphangiogenesis

Prox1 在胚胎和成人淋巴管生成中的作用

• 批准号: 7215718
• 负责人: NICOLE C JOHNSON
• 金额: $ 4.88万
• 依托单位: ST. JUDE CHILDREN'S RESEARCH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215718
• 关键词: Address; Adult; Cells; Complement; Condition; Defect; Development; Embryo; Endothelial Cells; Exhibits; Goals; Growth; Inflammation; Knock-out; Knowledge; Laboratories; Leg; Lymphangiogenesis; Lymphatic; Lymphatic Endothelial Cells; Lymphatic System; Lymphatic vessel; Mediating; Molecular; Mouse Strains; Mus; Newborn Infant; Pathologic; Phenotype; Proteins; Research Project Grants; Research Proposals; Role; Source; Staging; Standards of Weights and Measures; Stem cells; Time; Tissues; Venous; Wound Healing; postnatal

DESCRIPTION (provided by applicant): The long-term objective of this research proposal is to fully characterize the role of Proxl in embryonic and adult lymphangiogenesis. Our laboratory has previously shown that Proxl is critical for the development of the lymphatic vasculature and that Proxl heterozygous mice exhibit a mispatterned and dysfunctional lymphatic system. Furthermore, the fact that Proxl is also expressed in adult mouse lymphatic vessels suggested a likely functional role of Proxl in adult stage lymphagiogenesis in instances of wound healing and inflammation. My research project will strive to fully characterize the lymphatic defects seen in Proxl heterozygous mice using both standard knockout and inducible knockout mouse strains. A combination of cellular and molecular approaches will be used to explore the role of Proxl in both embryonic and adult lymphangiogenesis and to identify the different sources of lymphatic endothelial cell precursors. Taken together, these findings will further advance our knowledge of the developing lymphatic system.

描述（由申请人提供）：本研究计划的长期目标是充分表征 Proxl 在胚胎和成人淋巴管生成中的作用。我们的实验室此前已表明 Proxl 对于淋巴管系统的发育至关重要，并且 Proxl 杂合子小鼠表现出模式错误和功能失调的淋巴系统。此外，Proxl还在成年小鼠淋巴管中表达的事实表明Proxl可能在伤口愈合和炎症情况下的成年阶段淋巴管生成中发挥功能作用。我的研究项目将努力充分表征使用标准敲除和诱导敲除小鼠品系的 Proxl 杂合小鼠中观察到的淋巴缺陷。将结合细胞和分子方法来探索 Proxl 在胚胎和成人淋巴管生成中的作用，并鉴定淋巴内皮细胞前体的不同来源。总而言之，这些发现将进一步增进我们对发育中的淋巴系统的了解。