Regulation of RCC1 through N-terminal serine metnylation

通过 N 端丝氨酸甲基化调节 RCC1

基本信息

批准号：
7278264
负责人：
Christine E Schaner-Tooley
金额：
$ 4.88万
依托单位：
UNIVERSITY OF VIRGINIA
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-01 至 2008-12-10
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7278264
关键词：
Affect Affinity Antibodies Binding Biochemical Biological Assay Biological Process Cancer cell line Cell Cycle Cell physiology Chromatin Chromosome Segregation Cleaved cell Development Drug Delivery Systems Ensure Enzymes Fellowship Fractionation Guanine Guanine Nucleotide Exchange Factors Guanosine Triphosphate Phosphohydrolases Hela Cells In Vitro Individual Lead Localized Methionine Methods Methylation Methyltransferase Mitotic Checkpoint Mitotic spindle Modification Monomeric GTP-Binding Proteins N-terminal Names Nuclear Extract Nuclear Import Nucleotides Peptides Physical condensation Play Post-Translational Protein Processing Process Proteins Regulation Role Running Serine Small Interfering RNA Testing anti-cancer therapeutic carcinogenesis chemotherapy insight metaplastic cell transformation novel nucleocytoplasmic transport protein function research study segregation success therapeutic target

项目摘要

DESCRIPTION (provided by applicant): RCC1 is the nucleotide exchange factor for the small, Ras-like GTPase Ran. It is involved in many cellular processes including spindle assembly and spindle checkpoint regulation, that when misregulated can lead to cellular transformation and carcinogenesis. Determining how RCC1 is regulated will more clearly define how spindle assembly and checkpoint regulation are controlled and further the development of anticancer therapeutics targeted against these processes. A novel post-translational modification of RCC1, N-terminal serine methylation, has recently been identified in the Macara lab, and this proposal will examine its role in the regulation of RCC1 function. Specific aim #1 will determine which aspects of RCC1 function N-terminal serine methylation affects and how it regulates these functions. Specific aim #2 will identify the methyltransferase responsible for the N-terminal serine methylation of RCC1.

描述（由申请人提供）：RCC1 是小型 Ras 样 GTPase Ran 的核苷酸交换因子。它参与许多细胞过程，包括纺锤体组装和纺锤体检查点调节，当调节不当时会导致细胞转化和癌变。确定 RCC1 的调控方式将更清楚地定义纺锤体组装和检查点调控的控制方式，并进一步开发针对这些过程的抗癌疗法。 Macara 实验室最近发现了 RCC1 的一种新型翻译后修饰，即 N 端丝氨酸甲基化，该提案将研究其在 RCC1 功能调节中的作用。具体目标#1 将确定 N 端丝氨酸甲基化影响 RCC1 功能的哪些方面以及它如何调节这些功能。具体目标#2 将确定负责 RCC1 N 末端丝氨酸甲基化的甲基转移酶。