Development of olfactory glomeruli

嗅觉肾小球的发育

• 批准号: 7241598
• 负责人: PAUL FEINSTEIN
• 金额: $ 1.06万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7241598
• 关键词: Acids; Affect; Afferent Neurons; Alleles; Amino Acids; Animals; Anterior; Area; Axon; Binding; Brain region; Cilia; Code; Cyclic AMP; Development; Dissection; Epithelium; Family; Foundations; G-Protein-Coupled Receptors; GTP-Binding Proteins; Gene Expression; Gene Targeting; Genes; Genetic; Genetic Polymorphism; Inbred Strains Mice; Label; Lateral; Marriage; Medial; Modeling; Molecular; Mouse Strains; Mus; Neurons; Numbers; Odorant Receptors; Outcome; Pathway interactions; Pattern; Population; Positioning Attribute; Process; Publishing; Receptor Gene; Sorting - Cell Movement; Specificity; Structure; Surface; System; Testing; Time; Transmembrane Domain; axonal pathfinding; design; insight; neurodevelopment; neuronal cell body; olfactory bulb; receptor; receptor expression; research study; segregation

DESCRIPTION (provided by applicant): The coding of olfactory information begins with an animal's ability to detect and discriminate odorants. In the mouse olfactory system this is accomplished by a family ofapproximately 1000 functional odorant receptor genes each of which is expressed in thousands of neurons. A mature olfactory neuron expresses only 1 odorant receptor gene from only 1 allele. Odorant receptors (OR) are critical for the convergence of thousands of identical axons into 2 symmetrically located glomeruli on an olfactory bulb thereby creating approximately 2000 glomeruli. The marriage of mouse genetics and neural development has allowed for molecular dissection of these processes. This project continues with the analysis of the mouse olfactory system. In Aim 1, the temporal development will be determined for 2 highly related ORs that correspond to spatially related positions on the surface of the olfactory bulb. This analysis will determine if the onset of glomerular formation correlates with its final position on the olfactory bulb. A parallel study will determine if altering the number of axons for a given axonal population affects its position of convergence. In Aim 2, polymorphic OR proteins will probe if domains in the OR impart specificity to axons that converge into glomeruli. These experiments will provide a foundation for how olfactory glomeruli develop. Results generated will elaborate whether there are intrinsic positions on the olfactory bulb that are laid down by gradients and if there is an under appreciated plasticity in the olfactory system. The process by which olfactory sensory neurons are constantly re-establishing codes of connectivity to the olfactory bulb, may give insights into axonal pathfinding in other regions of the brain.

描述（由申请人提供）：嗅觉信息的编码始于动物检测和区分气味剂的能力。在小鼠嗅觉系统中，这是由1000个功能性气味受体基因的家族完成的，每个家族在数千个神经元中表达。成熟的嗅觉神经元仅表达1个来自1个等位基因的气味受体基因。气味受体（OR）对于数千个相同的轴突在嗅球上的对称位置肾小球的收敛至关重要，从而产生了大约2000个肾小球。小鼠遗传学和神经发育的婚姻允许这些过程的分子解剖。该项目继续对小鼠嗅觉系统进行分析。在AIM 1中，将针对与嗅球表面上的空间相关位置相对应的2个高度相关的OR确定时间发育。该分析将确定肾小球形成的开始是否与其在嗅球上的最终位置相关。一项并行研究将确定给定轴突种群的轴突数是否会影响其收敛位置。在AIM 2中，如果在或赋予对肾小球的轴突的特异性或赋予特异性，则多态性或蛋白质将探测。这些实验将为嗅觉肾小球的发展提供基础。产生的结果将详细说明嗅球上是否有固有位置由梯度放置，以及嗅觉系统中的可塑性是否不足。嗅觉感觉神经元不断重新建立与嗅球的连通性代码的过程可能会深入了解大脑其他区域的轴突途径。