Molecular analysis of Arabidopsis circadian regulation

拟南芥昼夜节律调节的分子分析

• 批准号: 7190513
• 负责人: Stacey L. Harmer
• 金额: $ 20.42万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7190513
• 关键词: Address; Affect; Alleles; Animal Model; Animals; Arabidopsis; Be++ element; Beryllium; Biochemical; Biochemical Genetics; Chromosome Mapping; Chromosomes; Circadian Rhythms; Cloning; Data; Disease; Elements; Eukaryota; Eukaryotic Cell; Flowers; Gene Expression; Gene Expression Regulation; Genes; Goals; Health; Hour; Human; Knowledge; Life; Maps; Molecular; Molecular Analysis; Molecular Genetics; Mouse-ear Cress; Nature; Nucleotides; Organism; Output; Phase; Phenotype; Photosynthesis; Physiological; Physiology; Placement; Plant Physiology; Plants; Protein Overexpression; Proteins; Regulation; Reporter Genes; Research; Role playing therapy; Rotation; Site-Directed Mutagenesis; System; Taxon; Techniques; Testing; Time; Transcription Coactivator; Vascular Plant; Work; base; biological adaptation to stress; day; environmental change; genetic analysis; improved; mutant; novel; positional cloning; promoter

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to better understand the molecular basis of circadian rhythms in eukaryotes. These studies will be carried out in Arabidopsis thaliana, a model organism well suited to genetic and biochemical approaches. Although putative clock components have been identified in Arabidopsis, the mechanism by which they regulate circadian rhythms is unclear. In addition, the means by which the central clock controls outputs so that they occur at the most advantageous time of day is unknown. The proposed studies use genetic and biochemical approaches to address these fundamental questions. First, a gene responsible for altered circadian regulation will be positionally cloned and other cloning projects will be initiated. Second, the mechanism by which the clock controls phase-specific gene regulation will be explored and a novel clock-regulated transcriptional activator identified. These studies will greatly improve our understanding of clock regulatory mechanisms, information that ultimately may be used to improve human health through treatment of circadian disorders.

描述（由申请人提供）：拟议研究的长期目标是更好地了解真核生物中昼夜节律的分子基础。这些研究将在拟南芥（Thaliana）中进行，这是一种非常适合遗传和生化方法的模型生物体。尽管已经在拟南芥中鉴定出推定的时钟成分，但是它们调节昼夜节律的机制尚不清楚。另外，中央时钟控制输出以使它们在一天中最有利的时间发生的均值尚不清楚。拟议的研究使用遗传和生化方法来解决这些基本问题。首先，将定位克隆负责改变昼夜节律法规的基因，并启动其他克隆项目。其次，将探索时钟控制相位基因调控的机制，并确定了新颖的时钟调节转录激活因子。这些研究将大大提高我们对时钟调节机制的理解，这些信息最终可以通过治疗昼夜节律来改善人类健康。