Center on Proteolytic Pathways (RMI)

蛋白水解途径中心 (RMI)

• 批准号: 6875817
• 负责人: Jeffrey W Smith
• 金额: $ 372.6万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-30 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6875817
• 关键词: bioengineering /biomedical engineering; biomedical resource; cooperative study; endopeptidases; enzyme activity; enzyme mechanism; gene expression profiling; imaging /visualization /scanning; mass spectrometry; proteolysis; proteomics; technology /technique development

DESCRIPTION (provided by applicant): Proteolysis is of paramount importance to biological regulation, and its significance is magnified because it is irreversible. Proteolysis regulates the four fundamental aspects of cell behavior; division, death, differentiation and motility. Proteolytic pathways are key to the pathology of virtually every type of human disease including, infection, inflammation, thrombosis, cancer, emphysema, Alzheimer's, etc. The importance and impact of understanding proteolysis "in total" will be immense. Achieving this ultimate goal will be a considerable challenge, and one that will require an ambitious and coordinated effort. Here we propose to form the Center on Proteolytic Pathways (CPP), a National Resource for the study of proteases, their inhibitors, their products, and upstream and downstream regulatory pathways. The strategic goal of the CPP is to develop The Protease Pathway Interrogation Platform (PPIP) technology. This platform technology will be comprised of four foundation technologies; (1) Activity-based Protease Profiling, (2) Protease Activity Imaging Technology, (3) Product Terminal Isotope Coding, and (4) the in silico environment called Protease Map. A key and unique feature of this technology platform is its focus squarely on measuring activity, as opposed to just abundance. The CPP will contain a Training component to ensure that a new cadre of scientists is trained to tackle protease biology with the most modem of techniques and most cutting edge technology. An Outreach component is planned to ensure that the CPP achieves high visibility, and ultimately numerous connections, with the scientific research community.

描述（由申请人提供）：蛋白水解对于生物调节至关重要，并且由于它是不可逆的，其重要性被放大。蛋白水解调节细胞行为的四个基本方面；分裂、死亡、分化和运动。蛋白水解途径是几乎所有类型人类疾病的病理学的关键，包括感染、炎症、血栓形成、癌症、肺气肿、阿尔茨海默病等。“全面”理解蛋白水解的重要性和影响将是巨大的。实现这一最终目标将是一项巨大的挑战，需要雄心勃勃且协调一致的努力。在这里，我们建议成立蛋白水解途径中心（CPP），作为研究蛋白酶、其抑制剂、其产品以及上下游调控途径的国家资源。 CPP 的战略目标是开发蛋白酶途径询问平台 (PPIP) 技术。该平台技术将由四项基础技术组成； (1) 基于活性的蛋白酶分析，(2) 蛋白酶活性成像技术，(3) 产品末端同位素编码，以及 (4) 称为蛋白酶图谱的计算机环境。该技术平台的一个关键而独特的特点是它专注于测量活动，而不是仅仅测量丰度。 CPP 将包含培训部分，以确保新的科学家骨干接受培训，能够使用最现代的技术和最尖端的技术来解决蛋白酶生物学问题。计划设立外展部分，以确保 CPP 获得较高的知名度，并最终与科学研究界建立广泛的联系。