Upgrade of a Campus NMR Facility for Biological Solids

校园生物固体核磁共振设施升级

基本信息

批准号：
7215520
负责人：
Leonard J Mueller
金额：
$ 27.75万
依托单位：
UNIVERSITY OF CALIFORNIA RIVERSIDE
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2009-03-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): We propose to upgrade an existing 600 MHz Bruker AVANCE liquid-state nuclear magnetic resonance spectrometer to perform NMR on biological solids. The current machine (1-year old) and the additionally requested equipment will become part of the University's Analytical Chemistry Instrumentation Facility (ACIF). Solid-state NMR has become an essential method of characterization for a wide range of medicinal systems in chemistry, biochemistry, materials science and biophysics. Its large impact within the University is witnessed by the diversity of projects put forth in this proposal. In particular, Michael Dunn and Len Mueller will study covalent intermediates bound to the ¿-site of tryptophan synthase, Michael Pirrung will characterize solid-phase synthetic oral insulin mimics, Katie DeFea and Len Mueller will determine the solid-state structure of PAR-2 agonists, and Chris Reed will investigate reactive cations in inorganic and organic chemistry. These problems all share a common need for solid-state characterization. Although well equipped for liquid-state NMR, there are currently no University-wide facilities capable of solid-state NMR at UCR. As such, solid-state NMR equipment will fill a noticeable void in the types of NMR characterization that can be performed at UC Riverside. Relevance: The relationship between structure and biological activity drives the development of therapeutic agents. Here we propose to add a significant molecular imaging capability through solid-state magnetic resonance. The goal is to determine the molecular level details that govern the interaction between drug and target molecules and build our chemical intuition for designing novel, next-generation treatments.

描述（由申请人提供）：我们建议升级现有的 600 MHz Bruker AVANCE 液态核磁共振波谱仪，以对生物固体进行 NMR 分析。现有机器（已使用 1 年）和额外要求的设备将成为现有机器的一部分。大学的分析化学仪器设备 (ACIF) 已成为化学、生物化学、材料领域广泛的药物系统表征的重要方法。该提案中提出的项目多样性证明了其在大学内的巨大影响，特别是迈克尔·邓恩（Michael Dunn）和伦·穆勒（Len Mueller）将研究与 ¿ 结合的共价中间体。 -色氨酸合酶位点，Michael Pirrung 将表征固相合成口服胰岛素模拟物，Katie DeFea 和 Len Mueller 将确定 PAR-2 激动剂的固态结构，Chris Reed 将研究无机和有机化学中的反应性阳离子所有问题都需要固态核磁共振，尽管加州大学河滨分校目前没有配备固态核磁共振的全校设施。核磁共振设备将填补加州大学河滨分校可进行的核磁共振表征类型的明显空白。相关性：结构和生物活性之间的关系推动了治疗药物的开发，我们的目标是确定控制药物和靶标之间相互作用的分子水平细节。分子并建立我们设计新颖的下一代治疗方法的化学直觉。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

10-Methyldodecanal, a Novel Attractant Pheromone Produced by Males of the South American Cerambycid Beetle Eburodacrys vittata.

10-甲基十二醛，一种由南美天牛 Eburodacrys vittata 雄性产生的新型引诱信息素。

DOI：
10.1371/journal.pone.0160727
发表时间：
2016
期刊：
PloS one
影响因子：
3.7
作者：
Silva,WelitonD;Millar,JocelynG;Hanks,LawrenceM;Bento,JoséMaurícioS
通讯作者：
Bento,JoséMaurícioS

Novel, male-produced aggregation pheromone of the cerambycid beetle Rosalia alpina, a priority species of European conservation concern.

DOI：
10.1371/journal.pone.0183279
发表时间：
2017
期刊：
PloS one
影响因子：
3.7
作者：
Žunič Kosi A;Zou Y;Hoskovec M;Vrezec A;Stritih N;Millar JG
通讯作者：
Millar JG

Chelation of a proton by an aliphatic tertiary diamine.

质子与脂肪族叔二胺的螯合。

DOI：
10.1021/ja8018725
发表时间：
2008
期刊：
Journal of the American Chemical Society
影响因子：
15
作者：
Yaghmaei,Sepideh;Khodagholian,Sevana;Kaiser,JMichael;Tham,FookS;Mueller,LeonardJ;Morton,ThomasHellman
通讯作者：
Morton,ThomasHellman

31P NMR investigation of backbone dynamics in DNA binding sites.

DNA 结合位点主链动力学的 31P NMR 研究。

DOI：
10.1021/jp711203m
发表时间：
2009
期刊：
The journal of physical chemistry. B
影响因子：
0
作者：
Tian,Ye;Kayatta,Michael;Shultis,Katharine;Gonzalez,Alejandro;Mueller,LeonardJ;Hatcher,MaryE
通讯作者：
Hatcher,MaryE