API 5000 LC/MS/MS System Package

API 5000 LC/MS/MS 系统套件

• 批准号: 7219842
• 负责人: Peter C Dedon
• 金额: $ 42.43万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7219842
• 关键词: Affect; Antineoplastic Agents; Area; Atmospheric Pressure; Biological; Biological Markers; Biomedical Engineering; Chemicals; Cities; Communities; DNA; Devices; Drug Design; Environmental Health; Faculty; Fostering; Foxes; Funding; Goals; Human; Infectious Agent; Inflammation; Lipids; Liver; Measures; Mechanical Stress; Methods; Proteins; Proteomics; Public Health; Range; Research; Research Project Grants; Scientist; Site; Specificity; Stress; System; Tissues; United States National Institutes of Health; base; bench to bedside; drug metabolism; human disease; human tissue; ion source; ionization; mass spectrometer; microbial alkaline proteinase inhibitor; response; systems research

DESCRIPTION (provided by applicant): We are requesting funds for the purchase a new API 5000 tandem quadrupole LC- MS/MS system, with turboionspray and atmospheric-pressure chemical ionization ion sources, manufactured by Applied Biosystems of Foster City, CA. The device will be used by the eight participating faculty, as well as other scientists in the MIT community, to quantify and characterize biological and synthetic molecules in studies affecting at least eighteen NIH-funded research projects. Our current single triple quadrupole mass spectrometer (API 3000) in the Center for Environmental Health Sciences (CEHS) is becoming overwhelmed by sharply increasing demand for LC-MS/MS analyses requiring greater sensitivity, mainly in the area of biomarker quantification. The co-applicants all have demonstrated needs for the API 5000 system for research projects funded by the NIH: Peter Dedon, John Essigmann, James Fox, Alan Grodzinsky, Leona Samson, David Schauer, Steven Tannenbaum, and John Wishnok. The projects that would benefit from the API 5000 range widely from proteomic analyses of cellular responses to infectious agents and mechanical stresses; anticancer drug design; drug metabolism in bioengineered liver tissue; to DNA, RNA, protein and lipid biomarkers of oxidative and nitrosative stress arising at sites of inflammation in humans. In all cases, the goal is to take advantage of the sensitivity and chemical specificity of the API 5000 triple quadrupole mass spectrometer system to rigorously quantify synthetic and biological molecules at the relatively low levels that occur in diseased and healthy human tissues, with sensitivities in the range of attomoles to femtomoles. No other system meets these demands. The public health relevance of the studies that would be supported by the API 5000 lies in their goals to develop sensitive methods to quantify molecules involved in both the therapy and the pathophysiological basis for human diseases. Sensitive measuring devices such as the API 5000 are crucial to the objective of moving NIH-sponsored research into humans - from bench to bedside.

描述（由申请人提供）：我们要求购买新的API 5000串联四极杆LC-MS/MS系统，其中包括涡轮增压和大气压化学离子化离子化来源，由加利福尼亚州福斯特市的应用生物系统制造。该设备将被八个参与的教师以及麻省理工学院社区的其他科学家使用，以量化和表征生物学和合成分子，这些研究影响至少18个NIH资助的研究项目。我们目前在环境健康科学中心（CEHS）中目前的单三极质谱仪（API 3000），由于对LC-MS/MS分析的需求急剧增加，主要需要更高的敏感性，主要是在生物标志物定量的领域中急剧增加。共同申请者都表明了对NIH资助的研究项目的API 5000系统的需求：Peter Dedon，John Essigmann，James Fox，Alan Grodzinsky，Leona Samson，David Schauer，Steven Tannenbaum和John Wishnok。将从API 5000中受益的项目范围广泛，范围从细胞对感染剂和机械应力的细胞反应的蛋白质组学分析范围广泛。抗癌药物设计；生物工程肝组织中的药物代谢；在人类炎症部位产生的氧化和亚硝化应激的DNA，RNA，蛋白质和脂质生物标志物。在所有情况下，目标是利用API 5000三倍四极杆质谱仪系统的敏感性和化学特异性，以严格量化患病和健康的人体组织中相对较低水平的合成和生物分子，在attomoles范围内对雌性组织具有敏感性。没有其他系统满足这些要求。 API 5000支持的研究的公共卫生相关性在于他们开发敏感方法来量化涉及人类疾病的疗法和病理生理基础的敏感方法。敏感的测量设备（例如API 5000）对于将NIH赞助的研究转移到人类的目的至关重要 - 从长椅到床边。