Applied Biosystems 7900HT Fast Real-Time PCR System

Applied Biosystems 7900HT 快速实时 PCR 系统

• 批准号: 7209700
• 负责人: GORDON S HUGGINS
• 金额: $ 15.89万
• 依托单位: TUFTS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-04 至 2008-06-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7209700
• 关键词: Blood Vessels; Cardiology; Cardiovascular Diseases; Cardiovascular system; Cause of Death; Disease; Equipment; Family; Funding; Gene Expression; Gene Mutation; Genes; Genotype; Goals; Health; Heart Diseases; High Blood Pressure; Housing; Individual; Laboratories; Life; Measures; Medical center; Methods; Molecular; Mutation; Myocardial Infarction; New England; Numbers; Patients; Pliability; Polymerase Chain Reaction; Productivity; Purpose; Reaction Time; Reagent; Research; Research Institute; Research Personnel; Robot; Running; Sampling; Stroke; System; Techniques; Testing; Time; United States; United States National Institutes of Health; Work; base; congenital heart disorder; cost; day; density; disability; human disease; improved; instrument; novel diagnostics; success; tool

DESCRIPTION (provided by applicant): Investigators in the Molecular Cardiology Research Institute (MCRI) at the Tufts-New England Medical Center are studying cardiovascular diseases with the goal of identifying new diagnostic tools and treatments that can be used to improve the treatment of heart disease patients. For example, several projects in the MCRI are seeking to identify abnormal genes that are responsible for cardiovascular diseases that run in families, such as high blood pressure and congenital heart disease. In addition to that work, a large fraction of NIH-funded MCRI Investigators, as well as other NIH-funded Investigators within the Tupper Research Building, wherein the MCRI is housed, currently examine the expression of genes to understand how these genetic changes occur with disease. Currently, these studies rely heavily on traditional methods that have a limited ability to accurately measure gene expression or offer low throughput analysis of gene mutations. Key to the success of gene and gene expression studies is equipment capable of reproducibly measuring levels of gene expression and gene mutations using preciously small amounts of starting material. For both gene and gene expression studies Real-Time PCR is now considered an essential technique. At this time, we are relying on individual investigators' real time PCR equipment that is outdated and without sufficient throughput or flexibility to meet the growing needs of our NIH- funded research. The purpose of this proposal, therefore, is to gain the support needed to purchase an Applied Biosystems 7900HT FAST Real-Time PCR System with Robot that can be used for gene expression and genotype applications that comprise the current NIH-funded research base described by the investigators of this proposal. First, automated sample handling by the machine's robot will allow for the analysis of thousands of genotype results quickly and efficiently. Second, by using the FAST capability of this machine PCR reaction times will be more rapid, allowing a greater number of users each day. Third, the ability to study 384 samples each time will enhance throughput while reducing reagent and labor costs. Finally, the ability to analyze multiple gene expression tests in a low-density array format would substantially enhance the throughput and flexibility of our work. Such an instrument is not currently available to the investigators taking part in this proposal. The unique advantages of the 7900HT Real-Time PCR System with Robot including FAST and low density array capability will accelerate the productivity of our laboratories, allowing greater opportunity to further the research of the investigators described in this proposal. Relevance to Human Disease: Diseases of the heart and blood vessels, including high blood pressure, stroke and heart attack, are the leading cause of death and disability in the United States. A 7900HT FAST Real-Time PCR System will allow investigators in the MCRI to accelerate their studies into how cardiovascular diseases can run in families and how changes in gene expression and function contribute to disease. The overall goal of these studies is to pursue new treatments for cardiovascular and other ailing diseases that will improve health and save lives.

描述（由申请人提供）：塔夫茨新英格兰医学中心分子心脏病学研究所（MCRI）的研究人员正在研究心血管疾病，目的是确定可用于改善心脏病患者治疗的新诊断工具和治疗方法。例如，MCRI中的几个项目正在寻求识别导致家庭中的心血管疾病的异常基因，例如高血压和先天性心脏病。除了这项工作外，很大一部分由NIH资助的MCRI研究者以及Tupper研究大楼内的NIH资助的其他研究人员，其中包括MCRI，目前正在研究基因的表达，以了解这些遗传变化是如何与疾病发生的。目前，这些研究在很大程度上依赖于传统方法，这些方法具有有限的准确测量基因表达或提供基因突变的吞吐量分析的能力有限。基因和基因表达研究成功的关键是能够使用少量的起始材料可重复测量基因表达和基因突变水平的设备。对于基因和基因表达研究，实时PCR现在被认为是必不可少的技术。目前，我们依靠单个调查人员的实时PCR设备，该设备已经过时并且没有足够的吞吐量或灵活性来满足我们NIH资助的研究的不断增长的需求。因此，该提案的目的是获得使用机器人购买应用的生物系统7900HT快速实时PCR系统所需的支持，该系统可用于基因表达和基因型应用，这些应用构成了该提案研究人员所描述的当前NIH资助的研究基础。首先，机器机器人的自动样品处理将允许快速有效地分析数千种基因型结果。其次，通过使用该机器的快速功能，PCR反应时间将更快，每天允许更多用户。第三，每次研究384个样本的能力将增强吞吐量，同时降低试剂和人工成本。最后，以低密度阵列格式分析多个基因表达测试的能力将大大提高我们工作的吞吐量和灵活性。目前，调查人员参与该提案的调查人员目前无法使用这种工具。具有机器人（包括快速和低密度阵列能力）的7900HT实时PCR系统的独特优势将加速我们实验室的生产率，从而使更多机会进一步进一步研究了本提案中所描述的研究人员的研究。与人类疾病有关：美国心脏和血管疾病，包括高血压，中风和心脏病发作，是美国的死亡和残疾的主要原因。 7900HT快速实时的PCR系统将使MCRI中的研究人员能够加速他们对家庭疾病如何在家庭中运作的研究以及基因表达和功能的变化如何对疾病有助于。这些研究的总体目标是为心血管和其他生病疾病寻求新的治疗方法，以改善健康并挽救生命。

项目成果

Association of OPRM1 and COMT single-nucleotide polymorphisms with hospital length of stay and treatment of neonatal abstinence syndrome.

• DOI: 10.1001/jama.2013.3411
• 发表时间: 2013-05-01
• 期刊: JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION
• 影响因子: 120.7
• 作者: Wachman, Elisha M.;Hayes, Marie J.;Brown, Mark S.;Paul, Jonathan;Harvey-Wilkes, Karen;Terrin, Norma;Huggins, Gordon S.;Aranda, Jacob V.;Davis, Jonathan M.
• 通讯作者: Davis, Jonathan M.