Macromolecular X-Ray Crystallography Instrument

高分子X射线晶体分析仪

• 批准号: 7214022
• 负责人: Neela H. Yennawar
• 金额: $ 50万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7214022
• 关键词: 15 year old; Address; Arts; Attenuated; Biochemistry; Biological Sciences; Complex; Core Facility; Crystallography; Data Collection; Data Set; Enzymes; Faculty; Funding; Goals; Health; Human poliovirus; Image; Institutes; Laboratories; Maintenance; Molecular Biology; Nucleic Acids; Numbers; Optics; Pennsylvania; Plant Proteins; Polioviruses; Polymerase; Proteins; RNA Viruses; Research; Research Personnel; Roentgen Rays; Screening procedure; Services; Speed; Technology; Time; United States National Institutes of Health; Universities; Viral Proteins; Week; X-Ray Crystallography; college; detector; insight; instrument; instrumentation; interest; member; mutant; vaccine development

DESCRIPTION (provided by applicant): The newly formed Macromolecular X-ray Crystallography Core Facility of the Pennsylvania State University seeks funds to purchase state-of-the-art X-ray instrumentation: Rigaku Micromax 007HF X-ray generator equipped with Varimax optics and R-AXIS HTC image plate detector. The center is one of the Shared Technology Facilities in the Huck Institute of the Life Sciences that will provide services to the entire University Park campus. Our existing Rigaku RU-200 X-ray generator is part of the Department of Biochemistry and Molecular Biology and is fifteen years old. Rigaku is currently having difficulty in getting parts for this instrument. Further, the flux of the existing generator is one-tenth that of the requested generator. The existing R-Axis IV and R-Axis IV++ detectors take a minimum of four and two minutes per image respectively. In reality typical crystal screening and data collection has taken two to three weeks in the past, limiting the use to about ten data sets between August and December of 2005. We need X-ray instrumentation with higher X-ray flux, faster data collection speed and easier maintenance. The new R-AXIS HTC detector is estimated to take a minimum of one minute per image. With the higher flux of Micromax 007HF, we expect the data collection times to reduce by a factor of four or more. With these advantages, we hope to meet the needs of the increasing number of investigators interested in incorporating macromolecular crystallography as part of their research. We have seventeen faculty members from two colleges and four departments participating in this proposal. Of these twelve are NIHfunded researchers and three have NIH proposals pending. The projects that will be addressed include viral proteins, plant proteins, complexes of proteins and nucleic acids and metallo-enzymes. Many of these will aid health related research. For example structural studies of poliovirus polymerase mutants could provide insight into attenuating RNA viruses for vaccine development. In conclusion, with the new instrumentation, our goal is to expand macromolecular crystallography research beyond just a few laboratories in the Department of Biochemistry and Molecular Biology.

描述（由申请人提供）：宾夕法尼亚州立大学的新成立的大分子X射线晶体学核心设施寻求资金购买最先进的X射线仪器：Rigaku Micromax 007HF X射线生成器，配备有varimax Optics和R-XAXIS HTC Image Plate Plate Plate Plate detector。该中心是哈克生命科学研究所的共享技术设施之一，它将为整个大学公园校园提供服务。我们现有的Rigaku Ru-200 X射线发电机是生物化学和分子生物学系的一部分，已有15岁了。 Rigaku目前很难为此乐器获取零件。此外，现有发电机的通量是所请求的发电机的十分之一。现有的R轴IV和R轴IV ++检测器分别为每个图像至少四分钟和两分钟。实际上，典型的晶体筛选和数据收集过去已花费了两到三个星期，将用途限制在2005年8月至12月之间的大约十个数据集。我们需要具有更高X射线通量，更快的数据收集速度和更容易维护的X射线仪器。据估计，新的R轴HTC检测器每图像至少需要一分钟。随着Micromax 007HF的较高通量，我们预计数据收集时间将减少四倍或更多。有了这些优势，我们希望满足有兴趣将大分子晶体学作为研究的一部分的研究人员的需求。我们有来自两个学院和四个部门的17名教职员工参加了这一建议。这十二个是NIH的研究人员，三名提出了NIH的建议。将要解决的项目包括病毒蛋白，植物蛋白，蛋白质和核酸的络合物以及金属酶。其中许多将有助于与健康有关的研究。例如，脊髓灰质炎病毒聚合酶突变体的结构研究可以洞悉减轻RNA病毒的疫苗发育。总之，借助新的仪器，我们的目标是将大分子晶体学研究扩展到生物化学和分子生物学系的少数实验室。

项目成果

Structural and Biochemical Characterizations of Methanoredoxin from Methanosarcina acetivorans, a Glutaredoxin-Like Enzyme with Coenzyme M-Dependent Protein Disulfide Reductase Activity.

来自 Methanosarcina acetivorans 的甲烷氧还蛋白的结构和生化特征，这是一种具有辅酶 M 依赖性蛋白二硫键还原酶活性的谷氧还蛋白样酶。

• DOI: 10.1021/acs.biochem.5b00823
• 发表时间: 2016
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Yenugudhati,Deepa;Prakash,Divya;Kumar,AdepuK;Kumar,RSivaSai;Yennawar,NeelaH;Yennawar,HemantP;Ferry,JamesG
• 通讯作者: Ferry,JamesG

Laser-improved protein crystallization screening.

激光改进的蛋白质结晶筛选。

• DOI: 10.1107/s1744309110023857
• 发表时间: 2010
• 期刊: Acta crystallographica. Section F, Structural biology and crystallization communications
• 作者: Yennawar,Neela;Denev,Sava;Gopalan,Venkataraman;Yennawar,Hemant
• 通讯作者: Yennawar,Hemant

Kinetic and structural characterization of dihydrofolate reductase from Streptococcus pneumoniae.

• DOI: 10.1021/bi901614m
• 发表时间: 2010-01-12
• 期刊: Biochemistry
• 影响因子: 2.9
• 作者: Lee J;Yennawar NH;Gam J;Benkovic SJ
• 通讯作者: Benkovic SJ