Acoustic detection of viruses bacteria and toxins

病毒、细菌和毒素的声学检测

• 批准号: 7577246
• 负责人: Matthew A Cooper
• 金额: $ 26.59万
• 依托单位: CAMBRIDGE MEDICAL INNOVATIONS LTD
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-10-18 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7577246
• 关键词: Acceleration; Acoustics; Antibodies; Antigens; Area; Bacteria; Binding; Biological Assay; Categories; Chemistry; Collaborations; Detection; Development; Devices; Enzyme-Linked Immunosorbent Assay; Feasibility Studies; Frequencies; Hand; Health; Housing; Human; Investigation; Label; Laboratories; Lead; Methods; National Institute of Allergy and Infectious Disease; Nature; Nucleic Acid Probes; Nucleic Acids; Nucleotides; Oligonucleotide Probes; Organism; Outcome; Performance; Polymerase Chain Reaction; Production; Proteins; Range; Research; Research Personnel; Specificity; Speed; Surface; Technology; Testing; Time; Toxin; Virus; Work; antigen binding; base; biodefense; instrumentation; miniaturize; particle; pathogen; prototype; rapid detection; receptor; sensor; small molecule; viral detection

DESCRIPTION (provided by investigators): Akubio, in collaboration with USAMRIID (Fort Detrick) intend to 1. Determine whether acoustic detection can differentiate between non-pathogenic and "priority" pathogenic organisms that are a threat to human health. An initial investigation into the use of acoustic detection for the rapid identification and quantization of a virus, a bacterium and a toxin, selected from the NIAID Category A, B or C list will be carried out. This work will establish performance baselines for the existing detection technology in the area of biodefense pathogen and toxin detection. 2. Apply acoustic detection to direct detection of pathogenic nucleic acid via hybridization of this material to specific oligonucleotide probes. The aim is to develop a direct, label-free method to detect pathogenic particles, antigens and nucleic acids simultaneously leading to a substantial reduction in assay time. 3. Develop multiplexed instrumentation and consumables to permit simultaneous testing for multiple agents. This will be achieved with an array of acoustic sensors used together, appropriate antibodies, and nucleic acid probes against a range of Category A, B or C pathogens. Profiles for antigen binding and nucleotide binding to appropriate receptors on the array will be obtained for specific pathogens. 4. Assess and establish the feasibility for production of a small portable device. The performance of this prototype will be compared rigorously with the laboratory-based instrumentation. A product specification and project plan for commercial development and manufacture of this hand-held unit will be produced.

描述（由调查人员提供）： Akubio 与 USAMRIID（德特里克堡）合作，打算 1. 确定声学检测是否可以区分对人类健康构成威胁的非致病性和“优先”致病性生物体。将针对使用声学检测来快速识别和量化从 NIAID A、B 或 C 类列表中选择的病毒、细菌和毒素进行初步研究。这项工作将为生物防御病原体和毒素检测领域的现有检测技术建立性能基线。 2. 通过将该材料与特定的寡核苷酸探针杂交，应用声学检测来直接检测病原性核酸。目的是开发一种直接、无标记的方法来同时检测致病颗粒、抗原和核酸，从而大幅缩短检测时间。 3. 开发复用仪器和消耗品，以允许同时测试多个试剂。这将通过一系列一起使用的声学传感器、适当的抗体和针对一系列 A、B 或 C 类病原体的核酸探针来实现。将获得特定病原体的与阵列上适当受体的抗原结合和核苷酸结合的概况。 4. 评估并确定生产小型便携式设备的可行性。该原型的性能将与实验室仪器进行严格比较。将制定用于商业开发和制造该手持设备的产品规格和项目计划。