Multiscale Engineering of Cell Scaffold for Bone Regeneration

骨再生细胞支架的多尺度工程

基本信息

批准号：
7248101
负责人：
Nicholas Alexander Kotov
金额：
$ 32.66万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-01 至 2011-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7248101
关键词：
Adhesions Affect Animal Testing Animals Architecture Biological Bone Marrow Bone Regeneration Bone Tissue Bone Transplantation Caliber Cell Adhesion Cell Density Cell-Matrix Junction Chemistry Coculture Techniques Condition Controlled Environment Cultured Cells Defect Degenerative Disorder Development Diffusion Disease Elasticity Engineering Facility Construction Funding Category Goals Growth Hydroxyapatites Immune Implant Infection Injury Life Mechanics Methods Military Personnel Modification Molds Morphology Natural regeneration Nature Nutrient Operative Surgical Procedures Osteoblasts Performance Polymers Porosity Predisposition Preparation Process Property Range Rate Research Research Personnel Risk SCID Mice Shapes Solutions Sports Stromal Cells Structure Surface Surface Properties Techniques Testing Tissue Donors Tissue Engineering Tissues Trauma War Weight-Bearing state age related allogenic bone transplantation base bone bone cell calcium phosphate calcium phosphate coating cell growth cell motility cellular engineering computer design concept design desire implantation improved in vivo migration nanocomposite nanoparticle nanoscale nanostructured polylactic acid-polyglycolic acid copolymer programs prototype scaffold subcutaneous tissue regeneration

项目摘要

DESCRIPTION (provided by applicant): Bone implants are in great demand for the treatment of for victims of trauma, military actions, congenital and degenerative diseases, as well as sports- and age-related injuries. The need for replacement tissue far outweighs the availability of donor tissue. The deficit is further exacerbated by war injuries. In this project we will target engineering of artificial bone implants that can potentially provide an endless supply of the bone material and eliminate the risk of immune rejection or disease transfer. Borrowing the concept from Nature, we propose to take advantage of multi-scale design of the bone scaffolds spanning the scale from 10-8 to 10-1 m. Multiple requirements of the artificial bone tissue, that include both mechanical and biological properties, make this approach acutely necessary. The following Specific Aims will be pursued to produce and implant prototype sustaining rapid development of bone cells, as well as high compression strengths. (1). Preparation of a biodegradable nanocomposite of PLGA and calcium phosphate (CP) nanoparticles with improved mechanical properties (10-8-10-6 m scale); (2). Construction of 3D biodegradable scaffold structure with inverted colloidal crystal (ICC) topography (10-4-10-6 m scale). (3) Increased cell attachment and growth by optimizing surface roughness through the application of nanoparticles to the scaffold surface (10-8-10-4 m scale); (4). Preparation of ICC scaffolds from PLGA-CP composite with a computer-designed cross-bar geometry (10-3-10-1 m scale); and (5). In vivo testing of the integrated scaffold with optimal geometry, mechanical properties, and surface roughness. These Specific Aims will allow us to obtain prototypes of implantable scaffolds, identify potential challenges and prove or disprove the principal concept of multi-scale design for artificial bone.

描述（由申请人提供）：骨植入物对于治疗创伤、军事行动、先天性和退行性疾病以及运动和年龄相关损伤的受害者有很大的需求。对替代组织的需求远远超过供体组织的可用性。战争伤害进一步加剧了赤字。在这个项目中，我们的目标是人造骨植入物的工程，它可以提供源源不断的骨材料供应，并消除免疫排斥或疾病转移的风险。借鉴自然的概念，我们建议利用骨支架的多尺度设计，其尺度从10-8到10-1 m。人造骨组织的多种要求，包括机械性能和生物性能，使得这种方法变得非常必要。将追求以下具体目标，以生产和植入维持骨细胞快速发育以及高压缩强度的原型。 (1).制备具有改善机械性能（10-8-10-6 m 尺度）的 PLGA 和磷酸钙（CP）纳米粒子的可生物降解纳米复合材料；（2）。构建具有倒置胶体晶体 (ICC) 形貌的 3D 可生物降解支架结构（10-4-10-6 m 尺度）。 (3) 通过在支架表面（10-8-10-4 m 尺度）应用纳米颗粒来优化表面粗糙度，从而增加细胞附着和生长；（4）。由 PLGA-CP 复合材料制备 ICC 支架，具有计算机设计的横杆几何形状（10-3-10-1 m 尺度）；和（5）。对具有最佳几何形状、机械性能和表面粗糙度的集成支架进行体内测试。这些具体目标将使我们能够获得可植入支架的原型，识别潜在的挑战，并证明或反驳人造骨多尺度设计的主要概念。