Multi-label Molecular FLIM of Breast Cancer

乳腺癌多标记分子FLIM

基本信息

批准号：
7281699
负责人：
JONATHAN QUINCY BROWN
金额：
$ 4.88万
依托单位：
DUKE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-09-01 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7281699
关键词：
Attention Binding Biological Markers Breast Cancer Model Breast Carcinoma Cells Contrast Media Cultured Cells Detection Diagnosis Diagnostic Discrimination Epidermal Growth Factor Receptor Epithelial Event Excision Exhibits Fatty acid glycerol esters Fellowship Fluorescence Goals Growth Factor Human Image Imagery Individual Injection of therapeutic agent Invasive Investigation Label Life MCF7 cell Malignant Neoplasms Mammary Neoplasms Metals Microscopic Microscopy Modality Modeling Molecular Molecular Models Molecular Target Monoclonal Antibodies Mus Names Nude Mice Numbers Optics Primary Neoplasm Protein Overexpression Research Solutions System Techniques Technology Time Tissues Transglutaminases Tumor Cell Line base fluorescence imaging fluorophore in vivo malignant breast neoplasm molecular imaging monolayer nanoparticle nanoshell outcome forecast prognostic quantum receptor function time use tissue/cell culture tool tumor

项目摘要

DESCRIPTION (provided by applicant): Optical molecular imaging of breast cancer using targeted fluorescent contrast agents has been demonstrated by several research groups; however, these applications generally involve imaging of single contrast agents. The objective of the proposed research is to enable non-invasive multi-label optical molecular imaging of multiple exogenous cancer markers, using fluorescence lifetime imaging (FLIM) to discriminate multiple contrast agents with overlapping emission spectra. The use of multi-label FLIM in molecular imaging has the potential to enable simultaneous imaging of many more contrast agents in vivo, which has important implications in the study, diagnosis, and prognosis of human breast cancer. Specific Aim I. Construct a model molecular system for multi-label FLIM and demonstrate feasibility of the approach in solution. Specific Aim II. Demonstrate selective targeting of contrast agents to multiple molecular breast cancer markers in cell culture, and demonstrate multi-label FLIM of the agents after binding with molecular targets. Specific Aim III. Perform the goals of Aim II in vivo in murine and human mammary tumors in the mouse.

描述（由申请人提供）：几个研究组证明了使用靶向荧光对比剂对乳腺癌进行光学分子成像。但是，这些应用通常涉及单个对比剂的成像。拟议的研究的目的是使用荧光寿命成像（FLIM）实现多个外源性癌症标志物的非侵入性多标签光学分子成像，以区分具有重叠发射光谱的多种对比度。在分子成像中使用多标签FLIM具有同时在体内进行更多对比剂的成像，这对人类乳腺癌的研究，诊断和预后具有重要意义。特定的目标I.构建一个模型分子系统，以进行多标签flim，并证明该方法在溶液中的可行性。具体目标II。证明对比度在细胞培养中选择性靶向多个分子乳腺癌标记物，并在与分子靶标结合后证明了药物的多标签效果。特定目标III。在小鼠中，在小鼠和人类乳腺肿瘤中执行AIM II的目标。