Core--Transgenic

核心--转基因

• 批准号: 7217715
• 负责人: Mieczyslaw Michael MICHALKIEWICZ
• 金额: $ 19.68万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217715
• 关键词: Lentivirus; angiotensin /renin /aldosterone hypertension; artificial chromosomes; biomedical facility; biotechnology; genetic manipulation; genetically modified animals; laboratory rat; transfection /expression vector

The Transgenic Core will provide the following services: 1) Generate transgenic rats with long inserts (>10 kb constructs or BACs) by pronuclear injection for Projects by Roman and Greene. 2) Generate specific lentiviral constructs for the production of transgenic rats as proposed in Project by Roman. 3) Efficiently generate large numbers of transgenic rats using lentiviral vectors for Project by Roman.

转基因核心将提供以下服务： 1）通过前注射长插入（> 10 kb构造或BAC）的转基因大鼠 罗马和格林的项目。 2）生成特定的慢病毒构建体，以生产罗马项目中提出的转基因大鼠。 3）使用慢病毒载体进行项目，有效地产生了大量的转基因大鼠。