Surgery, Physiology and Immunohistopathology

外科、生理学和免疫组织病理学

• 批准号: 7217647
• 负责人: Chris Glembotski
• 金额: $ 20.39万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217647
• 关键词: biomedical facility; cardiac myocytes; cardiovascular surgery; cell morphology; confocal scanning microscopy; disease /disorder model; echocardiography; fluorescence microscopy; genetically modified animals; histology; immunocytochemistry; immunopathology; laboratory mouse; molecular /cellular imaging; tissue /cell preparation

The Surgery, Physiology and Immunohistopathology Core (Core C), which will be used by all of the Projects of this PPG, will offer integrated services for assessing heart function and structure in settings that mimic cardiac pathology in mice. Accordingly, Core C will house and maintain the equipment and provide the staff necessary to offer Project Leaders and other PPG personnel the following services: 1) Surgery: in vivo myocardial infarction and transaortic constriction, 2) Physiology: echocardiography, Langendorff and working mouse hearts, 3) Immunohistopathology: state-of-the-art confocal fluorescent microscopic imaging of heart and myocyte structure, and cellular locations of signaling molecules in mouse heart sections and cultured cells. The Physiology, Surgery and Immunohistopathology Core, Core C, will be centrally located in the recently completed SDSL) BioScience Center, which houses the SDSU Heart Institute labs and administrative offices. Core C is composed of fully equipped surgery and physiology suites, dedicated mouse holding rooms, a complete tissue histology lab and a state-of-the-art confocal microscopy imaging facility. PPG investigators at all three institutions, as well as the live-cell imaging center in the Cell Biology Core, will be able to connect to the Core C imaging facility via an open microscopy environment (OME). The OME will optimize image data analysis, transfer and storage for all 5 of the PPG projects, as well as facilitating the integration of fixed sample imaging in Core C with live-cell imaging in Core B. An additional advantage of Core C is that it adjoins the SDSU Heart Institute Mouse Genetics Center, which facilitates the generation and use of genetically modified mice by all of the PPG projects. Core C was designed specifically to serve this multiinstitutional PPG; accordingly, it will maximize collaborations between projects, thus enhancing the synergy of this integrated research program. Core C complements and integrates with Core B by providing the ideal venue for examining cellular structure at the microscopic level, and by serving as the location where principles discovered using cells generated in the Cell Biology Core can be examined in a pathophysiological context that mimics the in vivo setting.

手术，生理学和免疫组织病理学核心（核心C）将由所有这些使用 该PPG的项目将提供综合服务，以评估心脏功能和结构 小鼠模仿心脏病理学。因此，核心C将容纳和维护设备并提供 为项目负责人和其他PPG人员提供以下服务所需的工作人员： 1）手术：体内心肌梗死和经跨动力收缩， 2）生理学：超声心动图，Langendorff和工作鼠标， 3）免疫组织病理学：最新的心脏和心肌的共聚焦荧光显微镜成像 小鼠心脏切片和培养细胞中信号分子的结构和细胞位置。 生理，手术和免疫组织病理学核心Core C将位于最近的 完成了SDSL）生物科学中心，该中心设有SDSU心脏研究所实验室和行政办公室。 Core C由设备齐全的手术和生理套件组成 完整的组织组织学实验室和最先进的共聚焦显微镜成像设施。 PPG调查人员 在所有三个机构以及细胞生物学核心中的活细胞成像中心都将能够连接 通过开放的显微镜环境（OME）到Core C成像设施。 OME将优化图像 所有5个PPG项目的数据分析，传输和存储，并促进固定的集成 核心C中的样品成像在核心B中具有活细胞成像。核心C的另一个优势是 毗邻SDSU心脏研究所鼠标遗传学中心，该中心有助于生成和使用 所有PPG项目都经过转基因小鼠。 Core C专门为这一多机构服务而设计 ppg;因此，它将最大化项目之间的合作，从而增强协同作用 这个集成的研究计划。核心C通过提供理想来补充并与核心B集成 在显微镜水平上检查细胞结构的场所，并通过作为位置 可以在病理生理学中检查使用细胞生物学核心中产生的细胞发现的原理 模仿体内设置的上下文。