Surgery, Physiology and Immunohistopathology

外科、生理学和免疫组织病理学

基本信息

批准号：
7217647
负责人：
Chris Glembotski
金额：
$ 20.39万
依托单位：
SAN DIEGO STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-07-01 至 2011-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7217647
关键词：
biomedical facility cardiac myocytes cardiovascular surgery cell morphology confocal scanning microscopy disease /disorder model echocardiography fluorescence microscopy genetically modified animals histology immunocytochemistry immunopathology laboratory mouse molecular /cellular imaging tissue /cell preparation

项目摘要

The Surgery, Physiology and Immunohistopathology Core (Core C), which will be used by all of the Projects of this PPG, will offer integrated services for assessing heart function and structure in settings that mimic cardiac pathology in mice. Accordingly, Core C will house and maintain the equipment and provide the staff necessary to offer Project Leaders and other PPG personnel the following services: 1) Surgery: in vivo myocardial infarction and transaortic constriction, 2) Physiology: echocardiography, Langendorff and working mouse hearts, 3) Immunohistopathology: state-of-the-art confocal fluorescent microscopic imaging of heart and myocyte structure, and cellular locations of signaling molecules in mouse heart sections and cultured cells. The Physiology, Surgery and Immunohistopathology Core, Core C, will be centrally located in the recently completed SDSL) BioScience Center, which houses the SDSU Heart Institute labs and administrative offices. Core C is composed of fully equipped surgery and physiology suites, dedicated mouse holding rooms, a complete tissue histology lab and a state-of-the-art confocal microscopy imaging facility. PPG investigators at all three institutions, as well as the live-cell imaging center in the Cell Biology Core, will be able to connect to the Core C imaging facility via an open microscopy environment (OME). The OME will optimize image data analysis, transfer and storage for all 5 of the PPG projects, as well as facilitating the integration of fixed sample imaging in Core C with live-cell imaging in Core B. An additional advantage of Core C is that it adjoins the SDSU Heart Institute Mouse Genetics Center, which facilitates the generation and use of genetically modified mice by all of the PPG projects. Core C was designed specifically to serve this multiinstitutional PPG; accordingly, it will maximize collaborations between projects, thus enhancing the synergy of this integrated research program. Core C complements and integrates with Core B by providing the ideal venue for examining cellular structure at the microscopic level, and by serving as the location where principles discovered using cells generated in the Cell Biology Core can be examined in a pathophysiological context that mimics the in vivo setting.

外科、生理学和免疫组织病理学核心（核心 C），将由所有该 PPG 项目将提供综合服务，用于评估心脏功能和结构：模拟小鼠的心脏病理学。因此，核心 C 将容纳和维护设备并提供为项目负责人和其他 PPG 人员提供以下服务所需的人员： 1）手术：体内心肌梗塞和经主动脉缩窄术， 2) 生理学：超声心动图、Langendorff 和工作小鼠心脏， 3) 免疫组织病理学：最先进的心脏和心肌细胞共聚焦荧光显微镜成像小鼠心脏切片和培养细胞中信号分子的结构和细胞位置。生理学、外科和免疫组织病理学核心（Core C）将位于最近的中心已竣工的 SDSL）生物科学中心，该中心设有 SDSU 心脏研究所实验室和行政办公室。 Core C 由设备齐全的手术和生理室、专用鼠标储藏室、完整的组织组织学实验室和最先进的共焦显微镜成像设施。 PPG调查员所有三个机构以及细胞生物学核心的活细胞成像中心都将能够连接通过开放式显微镜环境 (OME) 传输至 Core C 成像设施。 OME将优化图像所有 5 个 PPG 项目的数据分析、传输和存储，以及促进固定资产的集成 Core C 中的样品成像与 Core B 中的活细胞成像。Core C 的另一个优点是它毗邻 SDSU 心脏研究所小鼠遗传学中心，该中心有利于生成和使用所有 PPG 项目都对小鼠进行了基因改造。 Core C 是专门为服务这个多机构而设计的 PPG；因此，它将最大限度地提高项目之间的合作，从而增强协同作用这个综合研究计划。核心 C 通过提供理想的功能来补充和集成核心 B 在微观水平上检查细胞结构的场所，并作为使用细胞生物学核心中生成的细胞发现的原理可以在病理生理学中进行检查模仿体内环境的环境。