Service Core

服务核心

• 批准号: 7312554
• 负责人: A Vijaya RAO
• 金额: $ 28.99万
• 依托单位: UNIVERSITY OF TEXAS HLTH CTR AT TYLER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7312554
• 关键词: biomedical facility; biotechnology; fibrin; immunocytochemistry; in situ hybridization; morphometry; plasmin; plasminogen activator; plasminogen activator inhibitors; protein biosynthesis; protein engineering; protein purification; tissue /cell preparation; urokinase

Abnormalities of pathways of fibrinolysis have been implicated in the pathogenesis of pleural and acute lung injury (All). The objective of this PPG is to define novel mechanisms by which urokinase plasminogen activator (uPA), its receptor (uPAR), its inhibitor (plasminogen activator inhibitor-1, PAI-1) and its product (plasmin) influence the pathogenesis of All or pleural injury. To address this, the Project Leaders of this PPG will employ a number of various in vitro, in vivo and interventional methods, which require diverse proteins with highest purity and in large quantities. The Service Core is designed to provide this service. Further, all three projects of the PPG propose to use immunohistochemistry analysis to characterize the lung injury and expression of specific proteins/markers.relevant to lung injury and its repair. The Service Core provides the provision for such expertise. Thus, the aims of the Service Core are (i) to generate and provide various fibrinolytic proteins, their variants and derivatives, in sufficient quantities and purity to support the studies described in the individual projects comprising this PPG (ii) to provide support in pathology related services, including tissue preparation, thin sectioning, analysis of immunohistochemistry and in situ hybridization studies and morphometry. The Service Core uses proven technology for large-scale expression of recombinant fibrinolytic proteins and their variants. Efficient purification methods to purify recombinant proteins have been developed by either the project leaders or consultants of the PPG. A boardcertified Anatomic Pathologist with clinical and research expertise in thoracic pathology and application and interpretation of immunohistochemistry, in situ hybridization and morphometry provides expertise, accuracy and consistency in analysis of lung specimens. Overall, the Service Core provides a valuable support to all three projects of the PPG and maximizes the resources with substantial savings in cost.

纤溶途径异常与胸膜和急性肺损伤的发病机制有关（全部）。该 PPG 的目的是确定尿激酶纤溶酶原激活剂 (uPA)、其受体 (uPAR)、其抑制剂（纤溶酶原激活剂抑制剂-1、PAI-1）及其产物（纤溶酶）影响所有或胸膜损伤。为了解决这个问题，该 PPG 的项目负责人将采用多种体外、体内和介入方法，这些方法需要具有最高纯度和大量的多种蛋白质。服务核心旨在提供此服务。此外，PPG 的所有三个项目都建议使用免疫组织化学分析来表征肺损伤以及与肺损伤及其修复相关的特定蛋白质/标记物的表达。服务核心提供了此类专业知识。因此，服务核心的目标是 (i) 生成并提供足够数量和纯度的各种纤溶蛋白、其变体和衍生物，以支持包含本 PPG 的各个项目中描述的研究 (ii) 提供病理学支持相关服务，包括组织制备、薄片切片、免疫组织化学分析以及原位杂交研究和形态测量。服务核心采用成熟的技术大规模表达重组纤溶蛋白和 他们的变体。 PPG 的项目负责人或顾问开发了纯化重组蛋白的有效纯化方法。获得委员会认证的解剖病理学家，拥有胸部病理学以及应用和解释方面的临床和研究专业知识 免疫组织化学、原位杂交和形态测定提供了专业知识、准确性和 肺标本分析的一致性。总体而言，服务核心为 PPG 的所有三个项目提供了宝贵的支持，并最大限度地利用了资源，并大幅节省了成本。