LFMA: Microfluidics-Enabled Microarrays

LFMA：微流控微阵列

基本信息

批准号：
7272961
负责人：
Rajan Kumar
金额：
$ 20.59万
依托单位：
GENOME DATA SYSTEMS, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-01 至 2010-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7272961
关键词：
Address Apoptotic Biological Markers Cardiovascular Diseases Collaborations Complementary DNA Complex Condition DNA DNA Microarray Chip DNA Microarray format Development Device Designs Devices Diabetes Mellitus Diagnostic Dimensions Disease Dyes Future Gene Expression Genes Genome Glass Goals Health Height Human Kinetics Knowledge Label Laboratories Lateral Liquid substance MCF7 cell Malignant Neoplasms Measures Medicine Messenger RNA Methods Microarray Analysis Microfluidic Microchips Microfluidics Miniaturization Molecular Molecular Analysis Names Noise Performance Pharmaceutical Preparations Phase Printing Protocols documentation RNA Range Rate Reagent Sales Sampling Sensitivity and Specificity Services Signal Transduction Slide Spottings System Technology Testing Time Toxic Environmental Substances Transport Process Variant Video Microscopy attenuation cDNA Arrays design functional genomics genome sequencing improved instrument instrumentation novel strategies research study shear stress

项目摘要

DESCRIPTION (provided by applicant): This application proposes a novel approach to address the highly significant problem of microarray signal variation. Future advances in human health will that use knowledge gained from whole genome molecular analysis. Whole genome expression analysis using DNA microarrays has enabled rapid functional genomic studies and will provide the following significant benefits: discovery of new drugs to combat complex diseases such as cancer, diabetes and cardiovascular disorders, identification of new biomarkers leading to rapid molecular diagnostics and personalized medicine, and development of new strategies to identify and ameliorate effect of environmental toxins. However, current microarray technology has significant problems that limit their applicators. We propose to investigate improvements in microarray sensitivity and specificity using a method called Laminar flow Microarray Analysis (LFMA). This application has these specific aims 1) fabricate devices and systems that allow laminar flow studies of microarrays, 2) investigate effects of LFMA on microarray hybridization performance, and; 3) compare the performance of laminar flow hybridization with conventional static hybridization methods. We believe that successful development of LFMA will open the following commercial avenues for us: sale of disposable LFMA devices, automated instrument, and reagent kits, and provide laminar flow microarray analyses as a service. DNA microarray technology is a powerful method for whole genome expression analysis. After the completion of human genome sequencing, advances in human health will depend upon technologies that use knowledge gained from whole genome molecular analysis. Rapid functional genomic studies using microarrays will provide the following significant benefits: Discovery of new drugs to combat complex diseases such as cancer, diabetes and cardiovascular disorders; Identification of new biomarkers leading to rapid molecular diagnostics and personalized medicine; Development of new strategies to identify and ameliorate effect of environmental toxins.

描述（由申请人提供）：本申请提出了一种解决微阵列信号变化问题的新方法。人类健康的未来进步将使用从整个基因组分子分析中获得的知识。使用DNA微阵列的整个基因组表达分析已经实现了快速的功能基因组研究，并将提供以下重大好处：发现新药来打击诸如癌症，糖尿病和心血管疾病之类的复杂疾病，确定新的生物标志物，导致快速分子诊断的快速分子诊断以及个性化医学以及新的策略，以及新的策略，以及新的策略，以确定环境效果效应。但是，当前的微阵列技术存在限制其施用者的重大问题。我们建议使用称为层流微阵列分析（LFMA）的方法研究微阵列灵敏度和特异性的改进。该应用具有这些特定目的1）制造允许微阵列层流研究的设备和系统，2）研究LFMA对微阵列杂交性能的影响，以及； 3）比较层流杂交与常规静态杂交方法的性能。我们认为，LFMA的成功开发将为我们开放以下商业途径：销售一次性LFMA设备，自动化仪器和试剂盒，并提供层流流量微阵列分析作为服务。 DNA微阵列技术是整个基因组表达分析的强大方法。人类基因组测序完成后，人类健康的进步将取决于使用从整个基因组分子分析中获得的知识的技术。使用微阵列的快速功能性基因组研究将提供以下重大好处：发现新药来对抗癌症，糖尿病和心血管疾病等复杂疾病；鉴定新的生物标志物，导致快速分子诊断和个性化医学；制定识别和改善环境毒素作用的新策略。